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- W4310366818 abstract "Efficient methodologies have been empirically derived to accommodate the expedient isolation of RNA, techniques that should be scrutinized and refined continuously. Extraction tactics must be formulated to include sufficient management of RNase activity from all sources. Failure to scrupulously observe procedures for eliminating potential sources of RNase contamination will almost always yield a useless sample of degraded RNA. RNA lysis buffers are categorized as gentle if they leave the nucleus and other organelles intact, or chaotropic when they completely disrupt all organelles. Due to their naturally short half-life, experiments involving RNA are most judiciously planned around the availability of cell cultures or tissue samples and the actual date of RNA isolation. The seemingly endless list of permutations on a few fundamental RNA extraction techniques exist; as far as which RNA extraction procedure to use, the investigator should always think two steps ahead. RNA isolated from the nucleus or cytoplasm by direct cell lysis is known as steady-state RNA; it represents the final accumulation of the RNA in the cell or a subcellular compartment. Protocols for the analysis of nuclear RNA, including transcription rate assays, are presented in Chapter 19, Analysis of nuclear RNA. Most investigators have a strong preference for optimized kits for RNA purification, most of which are based on silica technology or magnetic separation. The isolation of miRNA, other noncoding RNAs, and RNA traveling in exosomes are similar to the standard chaotropic procedures for RNA isolation with the subtle modifications in the column binding and elution protocols." @default.
- W4310366818 created "2022-12-09" @default.
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- W4310366818 date "2023-01-01" @default.
- W4310366818 modified "2023-09-25" @default.
- W4310366818 title "RNA isolation strategies" @default.
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- W4310366818 doi "https://doi.org/10.1016/b978-0-323-90221-2.00042-4" @default.
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