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- W4310366842 abstract "Plants exhibit complex gene expression pathways and regulatory controls that are often similar to those observed in animals. Consequently, techniques that are commonly used to assay gene expression in animal cells may be used to assay the same in Plantae. The features of plant cells that distinguish them from animal cells include the presence of a cell wall, the presence of a large fluid-filled vacuole, and the presence of chloroplasts. The increased awareness and interest in agricultural biotechnology and applications thereof have created a need for expedient isolation of high-quality RNA from vastly different plant tissues, such as leaf, root, bark, floral tissue, fruit, and cotyledon. The fibrous nature of plant tissue often requires far more aggressive measures to disrupt cells than are required for an equal starting mass of animal tissue. The important consideration for isolating plant RNA is the method for dismantling or breaking through the formidable cell wall, methods include pulverizing plant tissue that has been frozen in liquid nitrogen, through the use of an electronic homogenizer, or by using a laboratory blender or similar apparatus. One of the primary complaints associated with RNA isolation from plant tissue is an unacceptably low yield because of the plant cell wall, high concentrations of phenolics, and high concentrations of polysaccharides, waxes, and latex. The amount of RNA that is to be expected is highly dependent upon the plant species, the specific tissue, and the isolation procedure. Plant RNA is remarkably stable when compared to the animal cell counterpart, meaning that there are often much greater opportunities for long-term storage and analysis." @default.
- W4310366842 created "2022-12-09" @default.
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- W4310366842 date "2023-01-01" @default.
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- W4310366842 title "Going green: RNA and the molecular biology of plants" @default.
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