FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310366881> ?p ?o ?g. }

• W4310366881 endingPage "349" @default.
• W4310366881 startingPage "301" @default.
• W4310366881 abstract "PCR is a primer-mediated, enzymatic amplification of specific genomic DNA or cDNA sequences. The sensitivity index ranking for transcription assays is based on three factors: intrinsic difficulty of the technique, reproducibility, and current average level of sensitivity. The final mass of the PCR product(s) is dependent on the efficiency of the reverse transcriptase and the efficiency of the thermostable enzyme(s) used to support PCR. Thus any reliable quantification method must factor in the relative efficiencies of both enzyme-mediated reactions because the overall efficiency of a technique is the product of the efficiencies of the individual steps involved. In “real-time” PCR, which is a fluorescence-based approach, PCR products are quantified as they accumulate, as opposed to performing a fixed number of cycles and then assessing the amount and variety of products by electrophoresis (end-point PCR). There are two ways to approach data analysis in real-time PCR applications. The first is the relative quantification method, whereby the amplification of an experimental template is compared with that of a control sample. The other method, known as absolute quantification, is used to calculate the actual copy number of template molecules with statistical confidence against a standard curve. The newest innovation in this area is known as digital PCR and a variant known as droplet digital PCR, methods which eliminate the need for standard curves and the calculation of threshold cycles. It is very important to include well-designed positive, negative, internal, and external controls in all quantitative and semiquantitative assays. Manuscripts submitted for publication should include information recommended by the MIQE guidelines so as to enable experimental reproducibility." @default.
• W4310366881 created "2022-12-09" @default.
• W4310366881 creator A5003710396 @default.
• W4310366881 date "2023-01-01" @default.
• W4310366881 modified "2023-10-18" @default.
• W4310366881 title "Quantitative PCR techniques" @default.
• W4310366881 cites W1547688219 @default.
• W4310366881 cites W1555442828 @default.
• W4310366881 cites W1559760142 @default.
• W4310366881 cites W1988966853 @default.
• W4310366881 cites W1994091239 @default.
• W4310366881 cites W1995301858 @default.
• W4310366881 cites W1996491588 @default.
• W4310366881 cites W2018686296 @default.
• W4310366881 cites W2022227920 @default.
• W4310366881 cites W2070721758 @default.
• W4310366881 cites W2094517427 @default.
• W4310366881 cites W2116211819 @default.
• W4310366881 cites W2128172592 @default.
• W4310366881 cites W2149897099 @default.
• W4310366881 cites W2165157887 @default.
• W4310366881 cites W2168420558 @default.
• W4310366881 cites W2579829632 @default.
• W4310366881 cites W3034862577 @default.
• W4310366881 cites W3118769644 @default.
• W4310366881 cites W3136916388 @default.
• W4310366881 doi "https://doi.org/10.1016/b978-0-323-90221-2.00037-0" @default.
• W4310366881 hasPublicationYear "2023" @default.
• W4310366881 type Work @default.
• W4310366881 citedByCount "0" @default.
• W4310366881 crossrefType "book-chapter" @default.
• W4310366881 hasAuthorship W4310366881A5003710396 @default.
• W4310366881 hasConcept C104317684 @default.
• W4310366881 hasConcept C156719811 @default.
• W4310366881 hasConcept C185592680 @default.
• W4310366881 hasConcept C43617362 @default.
• W4310366881 hasConcept C48023723 @default.
• W4310366881 hasConcept C48126324 @default.
• W4310366881 hasConcept C49105822 @default.
• W4310366881 hasConcept C54355233 @default.
• W4310366881 hasConcept C70721500 @default.
• W4310366881 hasConcept C70784841 @default.
• W4310366881 hasConcept C86803240 @default.
• W4310366881 hasConcept C9893847 @default.
• W4310366881 hasConceptScore W4310366881C104317684 @default.
• W4310366881 hasConceptScore W4310366881C156719811 @default.
• W4310366881 hasConceptScore W4310366881C185592680 @default.
• W4310366881 hasConceptScore W4310366881C43617362 @default.
• W4310366881 hasConceptScore W4310366881C48023723 @default.
• W4310366881 hasConceptScore W4310366881C48126324 @default.
• W4310366881 hasConceptScore W4310366881C49105822 @default.
• W4310366881 hasConceptScore W4310366881C54355233 @default.
• W4310366881 hasConceptScore W4310366881C70721500 @default.
• W4310366881 hasConceptScore W4310366881C70784841 @default.
• W4310366881 hasConceptScore W4310366881C86803240 @default.
• W4310366881 hasConceptScore W4310366881C9893847 @default.
• W4310366881 hasLocation W43103668811 @default.
• W4310366881 hasOpenAccess W4310366881 @default.
• W4310366881 hasPrimaryLocation W43103668811 @default.
• W4310366881 hasRelatedWork W1767130708 @default.
• W4310366881 hasRelatedWork W2151173819 @default.
• W4310366881 hasRelatedWork W2196023150 @default.
• W4310366881 hasRelatedWork W2331239048 @default.
• W4310366881 hasRelatedWork W2356023092 @default.
• W4310366881 hasRelatedWork W2895517696 @default.
• W4310366881 hasRelatedWork W2905706750 @default.
• W4310366881 hasRelatedWork W2957594148 @default.
• W4310366881 hasRelatedWork W3160513946 @default.
• W4310366881 hasRelatedWork W3193399059 @default.
• W4310366881 isParatext "false" @default.
• W4310366881 isRetracted "false" @default.
• W4310366881 workType "book-chapter" @default.