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- W4310366902 abstract "Microarrays are widely regarded both for direct comparison of two or more samples and as remarkable tools for high-throughput screening. A microarray, also known simply as an array or a gene chip, is typically a glass slide, a silicon wafer, or even a plastic substrate upon which very large numbers of individual gene sequences have been permanently applied. Microarrays were originally developed for the assay of mRNA expression from various biological sources. A newer generation of microarrays features silica beads retained in microwells and upon which multiple copies of oligonucleotides are attached. Candidate genes are generally identified by high-throughput microarray analysis; more refined quantitative gene expression data results from ensuing real-time PCR. Heat maps are one of the most useful methods for reporting data that often represent many samples, many genes, or both. A heat map is a graphical two-dimensional representation of microarray data in which the values of the data are represented in color. The most obvious advantage of microarray-based profiling of gene expression is the ability to assay very large numbers of genes in a single hybridization experiment. As a result of this rapid analysis of literally thousands of genes, microarray data are now being used to implicate specific genes and gene relays in the onset and progression of various diseases. The basic steps performed in a typical microarray analysis are acquisition of the microarray, isolation of high-quality RNA, cDNA synthesis, linkage of fluorescent dyes, purification of Cy-labeled probes, microarray hybridization, acquisition of data from the microarray slide or the filter, and determination of the biological significance and relevance of data. Not only are microarrays useful for gene expression profiling, but they are also valuable for mapping single nucleotide polymorphisms using a variety of detection innovations." @default.
- W4310366902 created "2022-12-09" @default.
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- W4310366902 date "2023-01-01" @default.
- W4310366902 modified "2023-09-27" @default.
- W4310366902 title "Array analysis of gene expression" @default.
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- W4310366902 doi "https://doi.org/10.1016/b978-0-323-90221-2.00036-9" @default.
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