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- W4310366914 abstract "The Northern analysis is the transfer or blotting, of electrophoretically separated RNAs from a gel to a filter membrane for subsequent fixation and hybridization. This chapter examines the options regarding the choice of transfer techniques, blotting membranes, and methods for nucleic acid fixation on the membrane. Northern analysis provides a qualitative and semiquantitative profile of the sample. It is compatible with RNA isolated from any source by any method and it continues to be a commonly used technique to assess mRNA length. The selection of the filter membrane to which electrophoretically resolved RNA is transferred prior to hybridization must be taken carefully. This selection must be influenced by the knowledge that different types of filters exhibit wide variations in nucleic acid binding capacity and support different transfer and immobilization methodologies. Nylon, polyvinylidene difluoride and, less frequently, nitrocellulose, are used as filter membranes and should be handled only with gloves and cut with sharp scissors or a scalpel. The actual transfer of electrophoretically separated RNA species from the gel to a solid support can be accomplished by capillary transfer, TurboBlotting, vacuum blotting, electroblotting, or alkaline blotting. Although filters can be stored confidently before hybridization, it is necessary to immobilize the sample on the filter by baking or UV crosslinking as soon as possible after transfer." @default.
- W4310366914 created "2022-12-09" @default.
- W4310366914 creator A5003710396 @default.
- W4310366914 date "2023-01-01" @default.
- W4310366914 modified "2023-09-23" @default.
- W4310366914 title "Northern analysis" @default.
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- W4310366914 doi "https://doi.org/10.1016/b978-0-323-90221-2.00022-9" @default.
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