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- W4310366955 abstract "The method of RNA in situ hybridization (RNA ISH) permits the observation of gene expression within the histological architecture of a specimen. RNA ISH is amenable to freshly isolated, flash-frozen tissue samples, as well as tissues that have been chemically crosslinked in either the recent or distant past. In situ hybridization is based on the same probe-target base-pairing principle that characterizes nearly all molecular biology methods; it alone, however, is the only method that precisely maps transcripts to specific cells within a tissue specimen. Information of this type can be of great importance in the realm of developmental biology as well as for the detection and staging of various diseases. The method is sufficiently sensitive to detect low-abundance transcripts. When performed with fluorescence-labeled probes of detection chemistries, the method is known as fluorescence in situ hybridization (FISH). Although it lacks the broad dynamic range associated with other methods including RT-PCR and RNA-seq, FISH signals can be boosted through the use of tyramide signal enhancement or branched DNA (bDNA) enhancement. There are well-respected immunochemical detection methods as well, including digoxigen and biotin. A variant of the RNA in situ method is a widely used cytogenetic diagnostic tool of localizing DNA targets on intact chromosomes." @default.
- W4310366955 created "2022-12-09" @default.
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- W4310366955 date "2023-01-01" @default.
- W4310366955 modified "2023-10-16" @default.
- W4310366955 title "RNA in situ hybridization" @default.
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- W4310366955 doi "https://doi.org/10.1016/b978-0-323-90221-2.00046-1" @default.
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