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- W4310367133 abstract "This chapter addresses issues unique to the isolation of RNA from animal tissue; the isolation of RNA from plant tissue is the focus of Chapter 7. The isolation of high-quality RNA from cells grown in culture is generally far easier to accomplish than is an attempted RNA isolation from tissue. The primary reason is that the three-dimensional architecture of the tissue must be dismantled and removed, compared to the disruption of a cell monolayer. Frequently, aggressive methods of homogenization in the presence of rather harsh formulations of lysis buffers are required. Some tissues are notorious for their legendary levels of RNase and reluctance to give up their RNA. Thus, the aggressive methods needed to compensate for the unique character of tissue often results in the isolation of total cellular RNA. Tissues can be snap-frozen, or otherwise stabilized and stored at −80°C immediately after harvesting. RNA isolation may then be performed at a more convenient time. RNA isolation from tissue is nonnegotiable when studying diseased tissue, when attempting to ascertain a tissue-specific response to an experimental challenge or another insult, or when trying to characterize the molecular physiology of normal tissue. In addition, the tissue sample itself may be difficult to acquire especially when the “phenomenon” is rare or when patient consent is required. Investigators working with human samples also know all too well the inherent dangers of working with dangerous infectious agents. In such cases, the mechanics of RNA isolation from tissue samples are modified compared to RNA isolation from cell culture samples for the safety of everyone. In addition, polysome isolation is a useful method for determining which mRNAs are actually ongoing translation, which is also a gene regulation control point." @default.
- W4310367133 created "2022-12-09" @default.
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- W4310367133 date "2023-01-01" @default.
- W4310367133 modified "2023-09-25" @default.
- W4310367133 title "The truth about tissues" @default.
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- W4310367133 doi "https://doi.org/10.1016/b978-0-323-90221-2.00008-4" @default.
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