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- W4310373532 abstract "The baculovirus expression vector system (BEVS) is a widely used platform for recombinant protein production for use in a wide variety of applications. Of particular interest is production of virus-like particles (VLPs), which consist of multiple viral proteins that self-assemble in strict stoichiometric ratios to mimic the structure of a virus but lacks its genetic material, while a significant amount of effort has been spent on optimizing expression ratios by co-infecting cells with multiple recombinant BEVs and modulating different process parameters, co-expressing multiple foreign genes from a single rBEV may offer more promise. However, there is currently a lack of promoters available with which to optimize co-expression of each foreign gene. To address this, previously published transcriptome data was used to identify promoters that have incrementally lower expression profiles and compared by expressing model cytoplasmic and secreted proteins. Bioinformatics was also used to identify sequence determinants that may be important for late gene transcription regulation, and translation initiation. The identified promoters and bioinformatics analyses may be useful for optimizing expression of foreign genes in the BEVS." @default.
- W4310373532 created "2022-12-09" @default.
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- W4310373532 date "2022-11-29" @default.
- W4310373532 modified "2023-09-25" @default.
- W4310373532 title "Utility of Alternative Promoters for Foreign Gene Expression Using the Baculovirus Expression Vector System" @default.
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- W4310373532 doi "https://doi.org/10.3390/v14122670" @default.
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