FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310478189> ?p ?o ?g. }

• W4310478189 endingPage "738" @default.
• W4310478189 startingPage "738" @default.
• W4310478189 abstract "To use hepatocytes immediately when necessary for hepatocyte transplantation and bioartificial liver (BAL) systems, a serum-free cryopreservation protocol ensuring the high survival of hepatocytes and maintenance of their functions should be developed. We established a serum-free protocol for the cryopreservation of primary hepatocytes, hepatocyte spheroids, and hepatocyte spheroid beads in liquid nitrogen. The serum-free cryopreservation solutions showed a significantly higher performance in maintaining enhanced viability and ammonia removal, urea secretion, and the albumin synthesis of hepatocyte spheroids and spheroid beads. The serum-free thawing medium, containing human serum albumin (HSA) and N-acetylcysteine (NAC), was compared with a fetal bovine serum-containing thawing medium for the development of a serum-free thawing medium. Our results show that hepatocyte spheroids and spheroid beads thawed using a serum-free thawing medium containing HSA and NAC exhibited increased hepatocyte viability, ammonia removal, urea secretion, and albumin synthesis compared to those thawed using the serum-containing medium. Finally, we evaluated the liver functions of the cryopreserved BAL system-applied serum-free cryopreservation process compared to the fresh BAL system. The ammonia removal efficiency of the cryopreserved hepatocyte spheroids BAL was lower than or similar to that of the fresh BAL system. Additionally, the urea concentrations in the media of all three BAL systems were not significantly different during BAL system operation. This cryopreserved spheroid-based BAL system using a serum-free process will be a good candidate for the treatment of patients." @default.
• W4310478189 created "2022-12-10" @default.
• W4310478189 creator A5001967366 @default.
• W4310478189 creator A5005608911 @default.
• W4310478189 creator A5010410639 @default.
• W4310478189 creator A5032840342 @default.
• W4310478189 creator A5035910796 @default.
• W4310478189 creator A5066965737 @default.
• W4310478189 creator A5077537019 @default.
• W4310478189 creator A5081756485 @default.
• W4310478189 creator A5088403655 @default.
• W4310478189 date "2022-11-29" @default.
• W4310478189 modified "2023-10-14" @default.
• W4310478189 title "Establishment of a Serum-Free Hepatocyte Cryopreservation Process for the Development of an “Off-the-Shelf” Bioartificial Liver System" @default.
• W4310478189 cites W159817623 @default.
• W4310478189 cites W1827766174 @default.
• W4310478189 cites W1858842697 @default.
• W4310478189 cites W1966201512 @default.
• W4310478189 cites W1967847140 @default.
• W4310478189 cites W1969001620 @default.
• W4310478189 cites W1978305805 @default.
• W4310478189 cites W1979869095 @default.
• W4310478189 cites W1981757033 @default.
• W4310478189 cites W1986376738 @default.
• W4310478189 cites W1988452786 @default.
• W4310478189 cites W1995324022 @default.
• W4310478189 cites W2007568976 @default.
• W4310478189 cites W2024007859 @default.
• W4310478189 cites W2025768402 @default.
• W4310478189 cites W2030780313 @default.
• W4310478189 cites W2033889537 @default.
• W4310478189 cites W2034751609 @default.
• W4310478189 cites W2046620120 @default.
• W4310478189 cites W2047239081 @default.
• W4310478189 cites W2049069390 @default.
• W4310478189 cites W2068963838 @default.
• W4310478189 cites W2071889935 @default.
• W4310478189 cites W2072835040 @default.
• W4310478189 cites W2074828925 @default.
• W4310478189 cites W2081207384 @default.
• W4310478189 cites W2082893364 @default.
• W4310478189 cites W2085993392 @default.
• W4310478189 cites W2086381108 @default.
• W4310478189 cites W2118681759 @default.
• W4310478189 cites W2148326484 @default.
• W4310478189 cites W2154234723 @default.
• W4310478189 cites W2157859754 @default.
• W4310478189 cites W2329358254 @default.
• W4310478189 cites W2334610317 @default.
• W4310478189 cites W2395116956 @default.
• W4310478189 cites W2624996598 @default.
• W4310478189 cites W4296816736 @default.
• W4310478189 doi "https://doi.org/10.3390/bioengineering9120738" @default.
• W4310478189 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36550944" @default.
• W4310478189 hasPublicationYear "2022" @default.
• W4310478189 type Work @default.
• W4310478189 citedByCount "1" @default.
• W4310478189 countsByYear W43104781892023 @default.
• W4310478189 crossrefType "journal-article" @default.
• W4310478189 hasAuthorship W4310478189A5001967366 @default.
• W4310478189 hasAuthorship W4310478189A5005608911 @default.
• W4310478189 hasAuthorship W4310478189A5010410639 @default.
• W4310478189 hasAuthorship W4310478189A5032840342 @default.
• W4310478189 hasAuthorship W4310478189A5035910796 @default.
• W4310478189 hasAuthorship W4310478189A5066965737 @default.
• W4310478189 hasAuthorship W4310478189A5077537019 @default.
• W4310478189 hasAuthorship W4310478189A5081756485 @default.
• W4310478189 hasAuthorship W4310478189A5088403655 @default.
• W4310478189 hasBestOaLocation W43104781891 @default.
• W4310478189 hasConcept C126322002 @default.
• W4310478189 hasConcept C16685009 @default.
• W4310478189 hasConcept C175369904 @default.
• W4310478189 hasConcept C179933525 @default.
• W4310478189 hasConcept C185592680 @default.
• W4310478189 hasConcept C196843134 @default.
• W4310478189 hasConcept C202751555 @default.
• W4310478189 hasConcept C2776125364 @default.
• W4310478189 hasConcept C2776200302 @default.
• W4310478189 hasConcept C2780365088 @default.
• W4310478189 hasConcept C2911091166 @default.
• W4310478189 hasConcept C55493867 @default.
• W4310478189 hasConcept C71867830 @default.
• W4310478189 hasConcept C71924100 @default.
• W4310478189 hasConcept C86803240 @default.
• W4310478189 hasConcept C86807702 @default.
• W4310478189 hasConcept C95444343 @default.
• W4310478189 hasConceptScore W4310478189C126322002 @default.
• W4310478189 hasConceptScore W4310478189C16685009 @default.
• W4310478189 hasConceptScore W4310478189C175369904 @default.
• W4310478189 hasConceptScore W4310478189C179933525 @default.
• W4310478189 hasConceptScore W4310478189C185592680 @default.
• W4310478189 hasConceptScore W4310478189C196843134 @default.
• W4310478189 hasConceptScore W4310478189C202751555 @default.
• W4310478189 hasConceptScore W4310478189C2776125364 @default.
• W4310478189 hasConceptScore W4310478189C2776200302 @default.
• W4310478189 hasConceptScore W4310478189C2780365088 @default.
• W4310478189 hasConceptScore W4310478189C2911091166 @default.
• W4310478189 hasConceptScore W4310478189C55493867 @default.

• next