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- W4310485183 abstract "The biofilm lifestyle plays a major role in the resistance and virulence of Pseudomonas aeruginosa and Enterococcus faecalis. In this study, two microencapsulated proteases (pepsin ME-PEP and trypsin ME-TRYP) were evaluated for their biofilm dispersal activity and their synergistic effect with microencapsulated carvacrol (ME-CARV). Spray-drying was used to protect enzymes and essential oil and enhance their activities. Cell count analysis proved the synergistic activity of enzymes and carvacrol treatment as biofilms were further reduced after combined treatment in comparison to ME-CARV or enzymes alone. Furthermore, results showed that sequential treatment in the order ME-TRYP - ME-PEP - ME-CARV resulted in more efficient biofilm removal with a maximum reduction of 5 log CFU mL-1 for P. aeruginosa and 4 log CFU mL-1 for E. faecalis. This study proposes that the combination of microencapsulated proteases with ME-CARV could be useful for the effective control of P. aeruginosa and E. faecalis biofilms." @default.
- W4310485183 created "2022-12-11" @default.
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- W4310485183 date "2022-10-21" @default.
- W4310485183 modified "2023-09-30" @default.
- W4310485183 title "Hurdle technology based on the use of microencapsulated pepsin, trypsin and carvacrol to eradicate <i>Pseudomonas aeruginosa</i> and <i>Enterococcus faecalis</i> biofilms" @default.
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- W4310485183 doi "https://doi.org/10.1080/08927014.2022.2151361" @default.
- W4310485183 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36451605" @default.
- W4310485183 hasPublicationYear "2022" @default.
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