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- W4310537524 abstract "Abstract Focal adhesions (FAs) strengthen their link with the actin cytoskeleton to resist force. Talin-vinculin association could reinforce actin anchoring to FAs by controlling actin polymerization. However, the actin polymerization activity of the talin-vinculin complex is not known because it requires the reconstitution of the mechanical and biochemical activation steps that control the association of talin and vinculin and the exposure of their actin-binding domains. By combining kinetic and binding assays with single actin filament observations in TIRF microscopy, we show that the association of talin and vinculin mutants, mimicking different degrees of activation, results in a variety of activities. In particular, mechanically stretched talin and activated vinculin combine to stimulate actin assembly synergistically through a sequential mechanism in which filaments are nucleated, capped and released to elongate. Our findings suggest a versatile mechanism for the regulation of actin assembly in FAs subjected to various combinations of biochemical and mechanical cues." @default.
- W4310537524 created "2022-12-11" @default.
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- W4310537524 date "2022-12-01" @default.
- W4310537524 modified "2023-10-18" @default.
- W4310537524 title "Talin and vinculin combine their activities to trigger actin assembly" @default.
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- W4310537524 doi "https://doi.org/10.1101/2022.12.01.518757" @default.
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