Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310542976> ?p ?o ?g. }
- W4310542976 endingPage "115000" @default.
- W4310542976 startingPage "115000" @default.
- W4310542976 abstract "In this study, a double-stranded DNA (dsDNA) fluorescent labeling method was developed using the fusion proteins of fluorescent protein (FP), and 7 kDa DNA-binding family members including Sso7d from Sulfolobus solfataricus, Aho7c from Acidianus hospitalis, ATSV7 from Acidianus tailed spindle virus and Sto7 from Sulfolobus tokodaii. Using this fluorescent DNA labeling method, we succeeded in single-molecule imaging of bacteriophage λDNA molecules stretched on glass surfaces. The fluorescence of the λDNA with FP fusion proteins decayed 2.4- to 6.4-fold slower than that of the typical intercalating method with SYTOX Green (SxG). In addition, the dynamic behaviors of FP-fused Aho7c-λDNA were relaxed and stretched with and without buffer flow, respectively, in microflow channels and were similar to that with typical intercalating dye, such as YOYO-1 and SxG. this fluorescent DNA labeling method. This fluorescent DNA labeling method can solve the problem of rapid fluorescence decay due to the intercalating dyes and therefore can be expected as an alternative to compound-based fluorescent dye. Thus, this study establishes FP fusion proteins as useful fluorescent DNA probes at the single-molecule level." @default.
- W4310542976 created "2022-12-11" @default.
- W4310542976 creator A5025631462 @default.
- W4310542976 creator A5034266812 @default.
- W4310542976 creator A5046562718 @default.
- W4310542976 creator A5054287751 @default.
- W4310542976 date "2023-02-01" @default.
- W4310542976 modified "2023-10-18" @default.
- W4310542976 title "A new fluorescence labeling method for molecular analysis of double-stranded DNA" @default.
- W4310542976 cites W1913922235 @default.
- W4310542976 cites W1964549900 @default.
- W4310542976 cites W1968253567 @default.
- W4310542976 cites W1975872313 @default.
- W4310542976 cites W1990621556 @default.
- W4310542976 cites W1996571717 @default.
- W4310542976 cites W2016225119 @default.
- W4310542976 cites W2040778984 @default.
- W4310542976 cites W2043280852 @default.
- W4310542976 cites W2046597732 @default.
- W4310542976 cites W2047083959 @default.
- W4310542976 cites W2050471630 @default.
- W4310542976 cites W2051602817 @default.
- W4310542976 cites W2056686563 @default.
- W4310542976 cites W2056782270 @default.
- W4310542976 cites W2070470763 @default.
- W4310542976 cites W2073193169 @default.
- W4310542976 cites W2085552659 @default.
- W4310542976 cites W2097131667 @default.
- W4310542976 cites W2101048694 @default.
- W4310542976 cites W2110272809 @default.
- W4310542976 cites W2163062162 @default.
- W4310542976 cites W2272847931 @default.
- W4310542976 cites W2333217077 @default.
- W4310542976 cites W2345910537 @default.
- W4310542976 cites W2554406865 @default.
- W4310542976 cites W2895692578 @default.
- W4310542976 cites W2913285065 @default.
- W4310542976 cites W2993396897 @default.
- W4310542976 cites W370906326 @default.
- W4310542976 cites W2026989301 @default.
- W4310542976 cites W3033826037 @default.
- W4310542976 doi "https://doi.org/10.1016/j.ab.2022.115000" @default.
- W4310542976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36470466" @default.
- W4310542976 hasPublicationYear "2023" @default.
- W4310542976 type Work @default.
- W4310542976 citedByCount "1" @default.
- W4310542976 countsByYear W43105429762023 @default.
- W4310542976 crossrefType "journal-article" @default.
- W4310542976 hasAuthorship W4310542976A5025631462 @default.
- W4310542976 hasAuthorship W4310542976A5034266812 @default.
- W4310542976 hasAuthorship W4310542976A5046562718 @default.
- W4310542976 hasAuthorship W4310542976A5054287751 @default.
- W4310542976 hasConcept C104317684 @default.
- W4310542976 hasConcept C121332964 @default.
- W4310542976 hasConcept C12554922 @default.
- W4310542976 hasConcept C153911025 @default.
- W4310542976 hasConcept C185592680 @default.
- W4310542976 hasConcept C2778644216 @default.
- W4310542976 hasConcept C2780631447 @default.
- W4310542976 hasConcept C2781287210 @default.
- W4310542976 hasConcept C550995028 @default.
- W4310542976 hasConcept C552990157 @default.
- W4310542976 hasConcept C55493867 @default.
- W4310542976 hasConcept C62520636 @default.
- W4310542976 hasConcept C86803240 @default.
- W4310542976 hasConcept C91881484 @default.
- W4310542976 hasConceptScore W4310542976C104317684 @default.
- W4310542976 hasConceptScore W4310542976C121332964 @default.
- W4310542976 hasConceptScore W4310542976C12554922 @default.
- W4310542976 hasConceptScore W4310542976C153911025 @default.
- W4310542976 hasConceptScore W4310542976C185592680 @default.
- W4310542976 hasConceptScore W4310542976C2778644216 @default.
- W4310542976 hasConceptScore W4310542976C2780631447 @default.
- W4310542976 hasConceptScore W4310542976C2781287210 @default.
- W4310542976 hasConceptScore W4310542976C550995028 @default.
- W4310542976 hasConceptScore W4310542976C552990157 @default.
- W4310542976 hasConceptScore W4310542976C55493867 @default.
- W4310542976 hasConceptScore W4310542976C62520636 @default.
- W4310542976 hasConceptScore W4310542976C86803240 @default.
- W4310542976 hasConceptScore W4310542976C91881484 @default.
- W4310542976 hasFunder F4320316429 @default.
- W4310542976 hasFunder F4320325454 @default.
- W4310542976 hasLocation W43105429761 @default.
- W4310542976 hasLocation W43105429762 @default.
- W4310542976 hasOpenAccess W4310542976 @default.
- W4310542976 hasPrimaryLocation W43105429761 @default.
- W4310542976 hasRelatedWork W1984878600 @default.
- W4310542976 hasRelatedWork W2014670920 @default.
- W4310542976 hasRelatedWork W2064953653 @default.
- W4310542976 hasRelatedWork W2078783484 @default.
- W4310542976 hasRelatedWork W2080571002 @default.
- W4310542976 hasRelatedWork W2096997987 @default.
- W4310542976 hasRelatedWork W2412503376 @default.
- W4310542976 hasRelatedWork W2768376329 @default.
- W4310542976 hasRelatedWork W4250392297 @default.
- W4310542976 hasRelatedWork W4310542976 @default.
- W4310542976 hasVolume "662" @default.
- W4310542976 isParatext "false" @default.