FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310544151> ?p ?o ?g. }

• W4310544151 abstract "Objectives: Successful tissue regeneration requires a clinically viable source of mesenchymal stem cells (MSCs). We explored activin receptor-like kinase (ALK)-5 inhibitors to rapidly derive an MSC-like phenotype with high cartilage forming capacity from a xeno-free human embryonic cell line. Methods: Embryonic stem cell (ESC) lines (H9 and HADC100) were treated with the ALK-5 inhibitor SB431542; HADC100 cells were additionally treated with ALK-5 inhibitors SB525334 or GW788388. Cells were then seeded upon human fibronectin in the presence of fibroblast growth factor 2 (FGF2) in a serum-free medium. Flow cytometry was used to assess MSC markers (positive for CD73, CD90, and CD105; negative for CD34 and CD45). Differentiation status was assessed through quantitative polymerase chain reaction. Cartilage forming capacity was determined in high-density pellet cultures, in fibrin gels containing extracellular matrix (fibrin-ECM), and after implantation in ex vivo human osteoarthritic cartilage. Gene expression, histology, and immunostaining were used to assess cartilage phenotype, tissue regeneration, and integration. Results: Exposure to all three ALK-5 inhibitors lead to expression of mesodermal gene markers and differentiation into MSC-like cells (embryonic stem cell-derived mesenchymal stem cells [ES-MSCs]) based on surface marker expression. ES-MSC in pellet cultures or in fibrin-ECM gels expressed high levels of chondrogenic genes: COL2A1, ACAN, and COMP; and low levels of COL1A1 and RUNX2. Cell pellets or fibrin constructs implanted into ex vivo human osteoarthritic cartilage defects produced GAG-rich (safranin O positive) and collagen type II-positive neocartilage tissues that integrated well with native diseased tissue. Conclusions: We developed a protocol for rapid differentiation of xeno-free ESC into MSC-like cells with high cartilage forming capacity with potential for clinical applications. Osteoarthritis (OA) is a common disease resulting in significant disability and no approved disease modifying treatment (other than total joint replacement). Embryonic stem cell-derived cell therapy has the potential to benefit patients with cartilage lesions leading to OA and may prevent or delay the need for total joint replacement." @default.
• W4310544151 created "2022-12-11" @default.
• W4310544151 creator A5022406593 @default.
• W4310544151 creator A5024487844 @default.
• W4310544151 creator A5056375701 @default.
• W4310544151 date "2023-01-27" @default.
• W4310544151 modified "2023-10-16" @default.
• W4310544151 title "ALK-5 Inhibitors for Efficient Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells" @default.
• W4310544151 cites W1536269141 @default.
• W4310544151 cites W1722374088 @default.
• W4310544151 cites W1905104097 @default.
• W4310544151 cites W1965276147 @default.
• W4310544151 cites W1969583812 @default.
• W4310544151 cites W1974384763 @default.
• W4310544151 cites W1982736212 @default.
• W4310544151 cites W1984965755 @default.
• W4310544151 cites W1999936525 @default.
• W4310544151 cites W2000965208 @default.
• W4310544151 cites W2002791185 @default.
• W4310544151 cites W2012593900 @default.
• W4310544151 cites W2015832973 @default.
• W4310544151 cites W2023748814 @default.
• W4310544151 cites W2025371271 @default.
• W4310544151 cites W2026757955 @default.
• W4310544151 cites W2031418162 @default.
• W4310544151 cites W2036519060 @default.
• W4310544151 cites W2037329204 @default.
• W4310544151 cites W2044113526 @default.
• W4310544151 cites W2046700486 @default.
• W4310544151 cites W2067163479 @default.
• W4310544151 cites W2074645865 @default.
• W4310544151 cites W2076914867 @default.
• W4310544151 cites W2085679263 @default.
• W4310544151 cites W2086943348 @default.
• W4310544151 cites W2090085208 @default.
• W4310544151 cites W2093369644 @default.
• W4310544151 cites W2097220529 @default.
• W4310544151 cites W2105045593 @default.
• W4310544151 cites W2113241496 @default.
• W4310544151 cites W2132950259 @default.
• W4310544151 cites W2138442302 @default.
• W4310544151 cites W2149831990 @default.
• W4310544151 cites W2158486555 @default.
• W4310544151 cites W2169962294 @default.
• W4310544151 cites W2191479718 @default.
• W4310544151 cites W2269013577 @default.
• W4310544151 cites W2428705285 @default.
• W4310544151 cites W2493782363 @default.
• W4310544151 cites W2498297759 @default.
• W4310544151 cites W2612859443 @default.
• W4310544151 cites W2901386638 @default.
• W4310544151 cites W2947958654 @default.
• W4310544151 cites W2957486497 @default.
• W4310544151 cites W2968327171 @default.
• W4310544151 cites W2990156135 @default.
• W4310544151 cites W2995298233 @default.
• W4310544151 cites W3028768728 @default.
• W4310544151 cites W3118990851 @default.
• W4310544151 cites W3119231555 @default.
• W4310544151 cites W3119587390 @default.
• W4310544151 cites W3132588466 @default.
• W4310544151 cites W3149714833 @default.
• W4310544151 cites W38879183 @default.
• W4310544151 cites W4311018638 @default.
• W4310544151 doi "https://doi.org/10.1089/ten.tea.2022.0164" @default.
• W4310544151 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36458467" @default.
• W4310544151 hasPublicationYear "2023" @default.
• W4310544151 type Work @default.
• W4310544151 citedByCount "0" @default.
• W4310544151 crossrefType "journal-article" @default.
• W4310544151 hasAuthorship W4310544151A5022406593 @default.
• W4310544151 hasAuthorship W4310544151A5024487844 @default.
• W4310544151 hasAuthorship W4310544151A5056375701 @default.
• W4310544151 hasConcept C10205521 @default.
• W4310544151 hasConcept C104317684 @default.
• W4310544151 hasConcept C105702510 @default.
• W4310544151 hasConcept C116716535 @default.
• W4310544151 hasConcept C136302470 @default.
• W4310544151 hasConcept C13685319 @default.
• W4310544151 hasConcept C145103041 @default.
• W4310544151 hasConcept C1491633281 @default.
• W4310544151 hasConcept C153911025 @default.
• W4310544151 hasConcept C185592680 @default.
• W4310544151 hasConcept C198826908 @default.
• W4310544151 hasConcept C20875687 @default.
• W4310544151 hasConcept C2780550940 @default.
• W4310544151 hasConcept C2780932548 @default.
• W4310544151 hasConcept C2781013607 @default.
• W4310544151 hasConcept C28328180 @default.
• W4310544151 hasConcept C35496256 @default.
• W4310544151 hasConcept C55493867 @default.
• W4310544151 hasConcept C86803240 @default.
• W4310544151 hasConcept C95444343 @default.
• W4310544151 hasConceptScore W4310544151C10205521 @default.
• W4310544151 hasConceptScore W4310544151C104317684 @default.
• W4310544151 hasConceptScore W4310544151C105702510 @default.
• W4310544151 hasConceptScore W4310544151C116716535 @default.
• W4310544151 hasConceptScore W4310544151C136302470 @default.
• W4310544151 hasConceptScore W4310544151C13685319 @default.
• W4310544151 hasConceptScore W4310544151C145103041 @default.

• next