FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310641812> ?p ?o ?g. }

• W4310641812 endingPage "74" @default.
• W4310641812 startingPage "61" @default.
• W4310641812 abstract "Abstract Some glycoproteins contain carbohydrates S-linked to cysteine (Cys) residues. However, relatively few S-glycosylated proteins have been detected, due to the lack of an effective research methodology. This work outlines a general concept for the detection of S-glycosylation sites in proteins. The approach was verified by exploratory experiments on a model mixture of β-S-glucosylated polypeptides obtained by the chemical transformation of lysozyme P00698. The model underwent two processes: (1) oxidative hydrolysis of S-glycosidic bonds under alkaline conditions to expose the thiol group of Cys residues; (2) thiol S-alkylation leading to thiol S-adduct formation at the former S-glycosylation sites. Oxidative hydrolysis was conducted in aqueous urea, dimethyl sulfoxide, or trifluoroethanol, with silver nitrate as the reaction promoter, in the presence of triethylamine and/or pyridine. The concurrent formation of stable protein silver thiolates, gluconic acid, and silver nanoclusters was observed. The essential de-metalation of protein silver thiolates using dithiothreitol preceded the S-labeling of Cys residues with 4-vinyl pyridine or a fluorescent reagent. The S-labeled model was sequenced by tandem mass spectrometry to obtain data on the modifications and their distribution over the protein chains. This enabled the efficiency of both S-glycosidic bonds hydrolysis and S-glycosylation site labeling to be evaluated. Suggestions are also given for testing this novel strategy on real proteomic samples." @default.
• W4310641812 created "2022-12-13" @default.
• W4310641812 creator A5053039302 @default.
• W4310641812 date "2022-12-02" @default.
• W4310641812 modified "2023-10-14" @default.
• W4310641812 title "Protein cysteine S-glycosylation: oxidative hydrolysis of protein S-glycosidic bonds in aqueous alkaline environments" @default.
• W4310641812 cites W1975130421 @default.
• W4310641812 cites W1976023732 @default.
• W4310641812 cites W1976538675 @default.
• W4310641812 cites W1978100568 @default.
• W4310641812 cites W1978895765 @default.
• W4310641812 cites W1983368897 @default.
• W4310641812 cites W1986956285 @default.
• W4310641812 cites W1987104478 @default.
• W4310641812 cites W1992292407 @default.
• W4310641812 cites W1994165491 @default.
• W4310641812 cites W2008819487 @default.
• W4310641812 cites W2019403411 @default.
• W4310641812 cites W2021108632 @default.
• W4310641812 cites W2024736595 @default.
• W4310641812 cites W2037560775 @default.
• W4310641812 cites W2040251840 @default.
• W4310641812 cites W2042178652 @default.
• W4310641812 cites W2044112601 @default.
• W4310641812 cites W2044744721 @default.
• W4310641812 cites W2051026192 @default.
• W4310641812 cites W2051797601 @default.
• W4310641812 cites W2053099367 @default.
• W4310641812 cites W2055019534 @default.
• W4310641812 cites W2056104886 @default.
• W4310641812 cites W2065110399 @default.
• W4310641812 cites W2067503835 @default.
• W4310641812 cites W2074716213 @default.
• W4310641812 cites W2075834182 @default.
• W4310641812 cites W2083169785 @default.
• W4310641812 cites W2084391380 @default.
• W4310641812 cites W2096666086 @default.
• W4310641812 cites W2102959758 @default.
• W4310641812 cites W2128625195 @default.
• W4310641812 cites W2155893773 @default.
• W4310641812 cites W2157147749 @default.
• W4310641812 cites W2166361259 @default.
• W4310641812 cites W2307033810 @default.
• W4310641812 cites W2332465486 @default.
• W4310641812 cites W2512633185 @default.
• W4310641812 cites W2522339646 @default.
• W4310641812 cites W2591000399 @default.
• W4310641812 cites W2614701602 @default.
• W4310641812 cites W2618574456 @default.
• W4310641812 cites W2682974080 @default.
• W4310641812 cites W2745131378 @default.
• W4310641812 cites W2746170656 @default.
• W4310641812 cites W2768346874 @default.
• W4310641812 cites W2782935800 @default.
• W4310641812 cites W2787783783 @default.
• W4310641812 cites W2788996531 @default.
• W4310641812 cites W2802626183 @default.
• W4310641812 cites W2885043570 @default.
• W4310641812 cites W2894992139 @default.
• W4310641812 cites W2918727817 @default.
• W4310641812 cites W2923713236 @default.
• W4310641812 cites W2971216353 @default.
• W4310641812 cites W3000263102 @default.
• W4310641812 cites W3029004374 @default.
• W4310641812 cites W3046990198 @default.
• W4310641812 cites W3083876407 @default.
• W4310641812 cites W3087237332 @default.
• W4310641812 cites W3088079530 @default.
• W4310641812 cites W3090230755 @default.
• W4310641812 cites W3091967242 @default.
• W4310641812 cites W3108954960 @default.
• W4310641812 cites W3127196630 @default.
• W4310641812 cites W3164729171 @default.
• W4310641812 cites W3194866316 @default.
• W4310641812 cites W3197824028 @default.
• W4310641812 cites W4283321869 @default.
• W4310641812 doi "https://doi.org/10.1007/s00726-022-03208-7" @default.
• W4310641812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36460841" @default.
• W4310641812 hasPublicationYear "2022" @default.
• W4310641812 type Work @default.
• W4310641812 citedByCount "0" @default.
• W4310641812 crossrefType "journal-article" @default.
• W4310641812 hasAuthorship W4310641812A5053039302 @default.
• W4310641812 hasBestOaLocation W43106418121 @default.
• W4310641812 hasConcept C150487720 @default.
• W4310641812 hasConcept C178790620 @default.
• W4310641812 hasConcept C181199279 @default.
• W4310641812 hasConcept C185592680 @default.
• W4310641812 hasConcept C2777313579 @default.
• W4310641812 hasConcept C55493867 @default.
• W4310641812 hasConceptScore W4310641812C150487720 @default.
• W4310641812 hasConceptScore W4310641812C178790620 @default.
• W4310641812 hasConceptScore W4310641812C181199279 @default.
• W4310641812 hasConceptScore W4310641812C185592680 @default.
• W4310641812 hasConceptScore W4310641812C2777313579 @default.
• W4310641812 hasConceptScore W4310641812C55493867 @default.
• W4310641812 hasFunder F4320322733 @default.
• W4310641812 hasIssue "1" @default.

• next