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- W4310662082 abstract "Equipment-free detection devices are attractive for point-of-care analysis, especially in resource-limited regions. Distancebased detection is the measurement of color change along the detection path related to the concentration of analytes. Hydrogels are three-dimensional crosslinked polymer networks with tunable properties and may be suitable for distance-based sensors. However, only small ions have been used as the targets in the previous studies. In this work, we demonstrated the possibility of using hydrogel as the distance-based measurement of large biomolecules such as proteins. The hydrogel was prepared with poly(ethylene glycol) diacrylate and a fluorescein derivative as the fluorescent detection probe. The fluorescent alteration in the presence of a target protein, trypsin, can be observed in both intensity-based and distance-based detection. It was found that the effect of the concentration of a fluorescent reagent was not significantly impacted in distance-based detection, while it is essential in intensity-based detection. The relationship between the distance change and the trypsin concentration in the range of 0.5 – 5 mM can be observed. This result showed the possibility of using hydrogel for distance-based detection of large biomolecules." @default.
- W4310662082 created "2022-12-15" @default.
- W4310662082 creator A5023096328 @default.
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- W4310662082 date "2023-02-20" @default.
- W4310662082 modified "2023-10-14" @default.
- W4310662082 title "Protein Determination by Distance and Color Changing <i>via</i> PEG-Based Hydrogels" @default.
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- W4310662082 doi "https://doi.org/10.15583/jpchrom.2022.015" @default.
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