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- W4310707316 abstract "Blood storage promotes the rapid depletion of red blood cell (RBC) high-energy adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (DPG), which are critical regulators of erythrocyte physiology and function, as well as oxygen kinetics and posttransfusion survival. Sphingosine-1-phosphate (S1P) promotes fluxes through glycolysis. We hypothesized that S1P supplementation to stored RBC units would improve energy metabolism and posttransfusion recovery. We quantified S1P in 1929 samples (n = 643, storage days 10, 23, and 42) from the REDS RBC Omics study. We then supplemented human and murine RBCs from good storer (C57BL6/J) and poor storer strains (FVB) with S1P (1, 5, and 10 μM) before measurements of metabolism and posttransfusion recovery. Similar experiments were repeated for mice with genetic ablation of the S1P biosynthetic pathway (sphingosine kinase 1 [Sphk1] knockout [KO]). Sample analyses included metabolomics at steady state, tracing experiments with 1,2,3-13C3-glucose, proteomics, and analysis of end-of-storage posttransfusion recovery, under normoxic and hypoxic storage conditions. Storage promoted decreases in S1P levels, which were the highest in units donated by female or older donors. Supplementation of S1P to human and murine RBCs boosted the steady-state levels of glycolytic metabolites and glycolytic fluxes, ie the generation of ATP and DPG, at the expense of the pentose phosphate pathway. Lower posttransfusion recovery was observed upon S1P supplementation. All these phenomena were reversed in Sphk1 KO mice or with hypoxic storage. S1P is a positive regulator of energy metabolism and a negative regulator of antioxidant metabolism in stored RBCs, resulting in lower posttransfusion recoveries in murine models." @default.
- W4310707316 created "2022-12-16" @default.
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- W4310707316 date "2023-04-12" @default.
- W4310707316 modified "2023-10-12" @default.
- W4310707316 title "Sphingosine 1-phosphate has a negative effect on RBC storage quality" @default.
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- W4310707316 cites W1501969485 @default.
- W4310707316 cites W1893921883 @default.
- W4310707316 cites W1966434594 @default.
- W4310707316 cites W1975728235 @default.
- W4310707316 cites W1984521724 @default.
- W4310707316 cites W2005252355 @default.
- W4310707316 cites W2013291317 @default.
- W4310707316 cites W2037372250 @default.
- W4310707316 cites W2042619626 @default.
- W4310707316 cites W2063106692 @default.
- W4310707316 cites W2067520432 @default.
- W4310707316 cites W2074089397 @default.
- W4310707316 cites W2087570879 @default.
- W4310707316 cites W2094600735 @default.
- W4310707316 cites W2141467202 @default.
- W4310707316 cites W2142862062 @default.
- W4310707316 cites W2149318847 @default.
- W4310707316 cites W2149902537 @default.
- W4310707316 cites W2149917889 @default.
- W4310707316 cites W2150319414 @default.
- W4310707316 cites W2157081117 @default.
- W4310707316 cites W2194318288 @default.
- W4310707316 cites W2300792970 @default.
- W4310707316 cites W2323043470 @default.
- W4310707316 cites W2327399609 @default.
- W4310707316 cites W2336431840 @default.
- W4310707316 cites W2461866271 @default.
- W4310707316 cites W2466448487 @default.
- W4310707316 cites W2469762698 @default.
- W4310707316 cites W2587940603 @default.
- W4310707316 cites W2668200717 @default.
- W4310707316 cites W2766399257 @default.
- W4310707316 cites W2766704126 @default.
- W4310707316 cites W2767265746 @default.
- W4310707316 cites W2796136485 @default.
- W4310707316 cites W2894355505 @default.
- W4310707316 cites W2896542487 @default.
- W4310707316 cites W2897068051 @default.
- W4310707316 cites W2898376167 @default.
- W4310707316 cites W2903145860 @default.
- W4310707316 cites W2921613748 @default.
- W4310707316 cites W2945041032 @default.
- W4310707316 cites W2946052571 @default.
- W4310707316 cites W2946569365 @default.
- W4310707316 cites W2965789264 @default.
- W4310707316 cites W2966593132 @default.
- W4310707316 cites W2971532346 @default.
- W4310707316 cites W2971797611 @default.
- W4310707316 cites W2981470702 @default.
- W4310707316 cites W2988667613 @default.
- W4310707316 cites W3002793417 @default.
- W4310707316 cites W3007643696 @default.
- W4310707316 cites W3011621712 @default.
- W4310707316 cites W3012615803 @default.
- W4310707316 cites W3015129246 @default.
- W4310707316 cites W3015536202 @default.
- W4310707316 cites W3044899919 @default.
- W4310707316 cites W3111424251 @default.
- W4310707316 cites W3118232987 @default.
- W4310707316 cites W3134872634 @default.
- W4310707316 cites W3143155190 @default.
- W4310707316 cites W3157833237 @default.
- W4310707316 cites W3159995035 @default.
- W4310707316 cites W3161401306 @default.
- W4310707316 cites W3161754417 @default.
- W4310707316 cites W3198675005 @default.
- W4310707316 cites W3212616106 @default.
- W4310707316 cites W3212952381 @default.
- W4310707316 cites W4220941514 @default.
- W4310707316 cites W4224989691 @default.
- W4310707316 cites W4283315622 @default.
- W4310707316 cites W4308773334 @default.
- W4310707316 doi "https://doi.org/10.1182/bloodadvances.2022008936" @default.
- W4310707316 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36469038" @default.
- W4310707316 hasPublicationYear "2023" @default.
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