Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310731964> ?p ?o ?g. }
- W4310731964 abstract "Microfluidic concentration gradient generators are useful in drug testing, drug screening, and other cellular applications to avoid manual errors, save time, and labor. However, expensive fabrication techniques make such devices prohibitively costly. Here, in the present work, we developed a microfluidic concentration gradient generator (μCGG) using a recently proposed non-conventional photolithography-less method. In this method, ceramic suspension fluid was shaped into a square mesh by controlling Saffman Taylor instability in a multiport lifted Hele-Shaw cell (MLHSC). Using the shaped ceramic structure as the template, μCGG was prepared by soft lithography. The concentration gradient was characterized and effect of the flow rates was studied using COMSOL simulations. The simulation result was further validated by creating a fluorescein dye (fluorescein isothiocanate) gradient in the fabricated μCGG. To demonstrate the use of this device for drug testing, we created various concentrations of an anticancer drug-curcumin-using the device and determined its inhibitory concentration on cervical cancer cell-line HeLa. We found that the IC50 of curcumin for HeLa matched well with the conventional multi-well drug testing method. This method of μCGG fabrication has multiple advantages over conventional photolithography such as: (i) the channel layout and inlet-outlet arrangements can be changed by simply wiping the ceramic fluid before it solidifies, (ii) it is cost effective, (iii) large area patterning is easily achievable, and (iv) the method is scalable. This technique can be utilized to achieve a broad range of concentration gradient to be used for various biological and non-biological applications." @default.
- W4310731964 created "2022-12-16" @default.
- W4310731964 creator A5017211248 @default.
- W4310731964 creator A5035647353 @default.
- W4310731964 creator A5039427502 @default.
- W4310731964 creator A5042892394 @default.
- W4310731964 creator A5043720339 @default.
- W4310731964 creator A5056084845 @default.
- W4310731964 creator A5079061757 @default.
- W4310731964 date "2022-12-01" @default.
- W4310731964 modified "2023-10-16" @default.
- W4310731964 title "Scalable large-area mesh-structured microfluidic gradient generator for drug testing applications" @default.
- W4310731964 cites W2015881798 @default.
- W4310731964 cites W2017549622 @default.
- W4310731964 cites W2018682190 @default.
- W4310731964 cites W2042002189 @default.
- W4310731964 cites W2064880903 @default.
- W4310731964 cites W2068141807 @default.
- W4310731964 cites W2078194899 @default.
- W4310731964 cites W2079230734 @default.
- W4310731964 cites W2093124126 @default.
- W4310731964 cites W2095788511 @default.
- W4310731964 cites W2112363756 @default.
- W4310731964 cites W2140210671 @default.
- W4310731964 cites W2161817427 @default.
- W4310731964 cites W2253660250 @default.
- W4310731964 cites W2338379031 @default.
- W4310731964 cites W2439612043 @default.
- W4310731964 cites W2555321517 @default.
- W4310731964 cites W2621425644 @default.
- W4310731964 cites W2769611623 @default.
- W4310731964 cites W2890893980 @default.
- W4310731964 cites W2914071992 @default.
- W4310731964 cites W2944972735 @default.
- W4310731964 cites W2977868568 @default.
- W4310731964 cites W3013452611 @default.
- W4310731964 cites W3097335977 @default.
- W4310731964 cites W4211041201 @default.
- W4310731964 doi "https://doi.org/10.1063/5.0126616" @default.
- W4310731964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36483022" @default.
- W4310731964 hasPublicationYear "2022" @default.
- W4310731964 type Work @default.
- W4310731964 citedByCount "2" @default.
- W4310731964 countsByYear W43107319642023 @default.
- W4310731964 crossrefType "journal-article" @default.
- W4310731964 hasAuthorship W4310731964A5017211248 @default.
- W4310731964 hasAuthorship W4310731964A5035647353 @default.
- W4310731964 hasAuthorship W4310731964A5039427502 @default.
- W4310731964 hasAuthorship W4310731964A5042892394 @default.
- W4310731964 hasAuthorship W4310731964A5043720339 @default.
- W4310731964 hasAuthorship W4310731964A5056084845 @default.
- W4310731964 hasAuthorship W4310731964A5079061757 @default.
- W4310731964 hasBestOaLocation W43107319642 @default.
- W4310731964 hasConcept C105487726 @default.
- W4310731964 hasConcept C118524077 @default.
- W4310731964 hasConcept C127413603 @default.
- W4310731964 hasConcept C136229726 @default.
- W4310731964 hasConcept C136525101 @default.
- W4310731964 hasConcept C142724271 @default.
- W4310731964 hasConcept C171250308 @default.
- W4310731964 hasConcept C192562407 @default.
- W4310731964 hasConcept C204787440 @default.
- W4310731964 hasConcept C41008148 @default.
- W4310731964 hasConcept C48044578 @default.
- W4310731964 hasConcept C71924100 @default.
- W4310731964 hasConcept C77088390 @default.
- W4310731964 hasConcept C8673954 @default.
- W4310731964 hasConceptScore W4310731964C105487726 @default.
- W4310731964 hasConceptScore W4310731964C118524077 @default.
- W4310731964 hasConceptScore W4310731964C127413603 @default.
- W4310731964 hasConceptScore W4310731964C136229726 @default.
- W4310731964 hasConceptScore W4310731964C136525101 @default.
- W4310731964 hasConceptScore W4310731964C142724271 @default.
- W4310731964 hasConceptScore W4310731964C171250308 @default.
- W4310731964 hasConceptScore W4310731964C192562407 @default.
- W4310731964 hasConceptScore W4310731964C204787440 @default.
- W4310731964 hasConceptScore W4310731964C41008148 @default.
- W4310731964 hasConceptScore W4310731964C48044578 @default.
- W4310731964 hasConceptScore W4310731964C71924100 @default.
- W4310731964 hasConceptScore W4310731964C77088390 @default.
- W4310731964 hasConceptScore W4310731964C8673954 @default.
- W4310731964 hasFunder F4320334771 @default.
- W4310731964 hasIssue "6" @default.
- W4310731964 hasLocation W43107319641 @default.
- W4310731964 hasLocation W43107319642 @default.
- W4310731964 hasLocation W43107319643 @default.
- W4310731964 hasOpenAccess W4310731964 @default.
- W4310731964 hasPrimaryLocation W43107319641 @default.
- W4310731964 hasRelatedWork W1817510052 @default.
- W4310731964 hasRelatedWork W2021077848 @default.
- W4310731964 hasRelatedWork W2128858074 @default.
- W4310731964 hasRelatedWork W2186999538 @default.
- W4310731964 hasRelatedWork W2188851458 @default.
- W4310731964 hasRelatedWork W2340465956 @default.
- W4310731964 hasRelatedWork W2532096506 @default.
- W4310731964 hasRelatedWork W2795577310 @default.
- W4310731964 hasRelatedWork W3127702166 @default.
- W4310731964 hasRelatedWork W4381486281 @default.
- W4310731964 hasVolume "16" @default.
- W4310731964 isParatext "false" @default.