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- W4310778877 abstract "Thioflavin T, a cationic benzothiazole dye, is typically used to detect amyloid fibrils. In this study, we analyzed the staining properties of Bacillus subtilis cells using several fluorescent dyes, including thioflavin T analogs, 2-(4'-methylaminophenyl) benzothiazole (BTA-1), and 2-(4-aminophenyl) benzothiazole (APBT). Thioflavin T stained vegetative cells in the early log phase and outer layer structures of forespores and mature spores. The inner parts of forespores and heat-killed mature spores were also stained with thioflavin T. Congo red, auramine O, and rhodamine B stained forespores and mature spores similar to thioflavin T. In contrast, APBT and BTA-1 fluorescence was detected in the outer layers of vegetative cells, mother cells, forespores, and mature spores, indicating that they bind to the cell membrane and/or cell wall. The combination of the fluorescent dyes used in this study will help analyze morphogenetic processes during the sporulation and the damage mechanisms of vegetative cells and spores." @default.
- W4310778877 created "2022-12-17" @default.
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- W4310778877 date "2022-12-06" @default.
- W4310778877 modified "2023-10-01" @default.
- W4310778877 title "Comparative analysis of thioflavin T and other fluorescent dyes for fluorescent staining of<i>Bacillus subtilis</i>vegetative cell, sporulating cell, and mature spore" @default.
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- W4310778877 doi "https://doi.org/10.1093/bbb/zbac195" @default.
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