FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310817650> ?p ?o ?g. }

• W4310817650 abstract "Abstract Objective: The purpose of the present study was to explore the transcriptome differences of sheep in IVF. Embryos were fertilized in vitro at different developmental stages in order to assess the differences of the function, classification and metabolic pathway of differentially expressed genes and to provide a theoretical basis for revealing the regulatory mechanism of sheep early embryo development. The experiments aimed to promote the development of sheep embryo production technology in vitro. Methods: The single embryos derived from sheep 16-cells and morulae were produced by IVF technology as samples and the sequencing library was constructed by the Smart-Seq2 amplification technology. The transcriber was sequenced by Illumina HiSeqXten high-throughput sequencing technology and the effective sequences were analyzed by functional annotation and related bioinformatic analysis. Results: The results indicated that the Clean reads of 16-cell and morula embryos were 47355570-50855888, of which 93.17%-94.23% reads were compared with the reference genome sequence of sheep; 10 were compared with alternative splicing types of transcription terminal site (TTS) and with the transcription start site (TSS), which accounted for the largest proportion in sheep embryo transcripts. There were 171170-211487 sites of single nucleotide polymorphisms (SNPs) and 9802-10505 sites of novel transcripts in sheep 16-cells and morulas respectively. A total of 10 genes (including novel transcripts) were expressed at the morula stage.The following screening criteria were used: | log2ratio |≥1 and Q-value < 0.05. The differences in the 17,607 DEGs between the 16-cell and morula embryos were significant (370 upregulated and 47 downregulated). From the 16-cell stage to the morula stage, 16,343 DEGs were classified and annotated. It was found that the metabolic processes of BP, CC and MF3 focused on biological functions, such as organic synthesis and nucleic acid synthesis. KEGG analysis indicated 206 pathways that were involved in the 16-cell to morula embryo stage. Two pathways were used for enrichment namely the spliceosome and the signaling pathways regulating pluripotency of stem cells, respectively. Conclusions: The number of differentially expressed genes was identified at different stages of sheep embryo development and the function, classification and metabolic pathway of differentially expressed genes were obtained." @default.
• W4310817650 created "2022-12-18" @default.
• W4310817650 creator A5002233110 @default.
• W4310817650 creator A5015105154 @default.
• W4310817650 creator A5031315906 @default.
• W4310817650 creator A5056654754 @default.
• W4310817650 creator A5062273391 @default.
• W4310817650 creator A5084476050 @default.
• W4310817650 date "2022-12-07" @default.
• W4310817650 modified "2023-10-05" @default.
• W4310817650 title "Transcriptome Analysis of Sheep IVF embryos based on single Cell RNA-Seq" @default.
• W4310817650 doi "https://doi.org/10.21203/rs.3.rs-375410/v1" @default.
• W4310817650 hasPublicationYear "2022" @default.
• W4310817650 type Work @default.
• W4310817650 citedByCount "0" @default.
• W4310817650 crossrefType "posted-content" @default.
• W4310817650 hasAuthorship W4310817650A5002233110 @default.
• W4310817650 hasAuthorship W4310817650A5015105154 @default.
• W4310817650 hasAuthorship W4310817650A5031315906 @default.
• W4310817650 hasAuthorship W4310817650A5056654754 @default.
• W4310817650 hasAuthorship W4310817650A5062273391 @default.
• W4310817650 hasAuthorship W4310817650A5084476050 @default.
• W4310817650 hasBestOaLocation W43108176501 @default.
• W4310817650 hasConcept C104317684 @default.
• W4310817650 hasConcept C105580179 @default.
• W4310817650 hasConcept C106337198 @default.
• W4310817650 hasConcept C107397762 @default.
• W4310817650 hasConcept C135763542 @default.
• W4310817650 hasConcept C141231307 @default.
• W4310817650 hasConcept C150194340 @default.
• W4310817650 hasConcept C153209595 @default.
• W4310817650 hasConcept C162317418 @default.
• W4310817650 hasConcept C16685009 @default.
• W4310817650 hasConcept C194583182 @default.
• W4310817650 hasConcept C196843134 @default.
• W4310817650 hasConcept C50489715 @default.
• W4310817650 hasConcept C54355233 @default.
• W4310817650 hasConcept C70721500 @default.
• W4310817650 hasConcept C71924100 @default.
• W4310817650 hasConcept C86803240 @default.
• W4310817650 hasConceptScore W4310817650C104317684 @default.
• W4310817650 hasConceptScore W4310817650C105580179 @default.
• W4310817650 hasConceptScore W4310817650C106337198 @default.
• W4310817650 hasConceptScore W4310817650C107397762 @default.
• W4310817650 hasConceptScore W4310817650C135763542 @default.
• W4310817650 hasConceptScore W4310817650C141231307 @default.
• W4310817650 hasConceptScore W4310817650C150194340 @default.
• W4310817650 hasConceptScore W4310817650C153209595 @default.
• W4310817650 hasConceptScore W4310817650C162317418 @default.
• W4310817650 hasConceptScore W4310817650C16685009 @default.
• W4310817650 hasConceptScore W4310817650C194583182 @default.
• W4310817650 hasConceptScore W4310817650C196843134 @default.
• W4310817650 hasConceptScore W4310817650C50489715 @default.
• W4310817650 hasConceptScore W4310817650C54355233 @default.
• W4310817650 hasConceptScore W4310817650C70721500 @default.
• W4310817650 hasConceptScore W4310817650C71924100 @default.
• W4310817650 hasConceptScore W4310817650C86803240 @default.
• W4310817650 hasLocation W43108176501 @default.
• W4310817650 hasOpenAccess W4310817650 @default.
• W4310817650 hasPrimaryLocation W43108176501 @default.
• W4310817650 hasRelatedWork W1976892151 @default.
• W4310817650 hasRelatedWork W2177113349 @default.
• W4310817650 hasRelatedWork W2268123450 @default.
• W4310817650 hasRelatedWork W2327861445 @default.
• W4310817650 hasRelatedWork W2396424000 @default.
• W4310817650 hasRelatedWork W2463742850 @default.
• W4310817650 hasRelatedWork W2791583078 @default.
• W4310817650 hasRelatedWork W2951586855 @default.
• W4310817650 hasRelatedWork W2990525442 @default.
• W4310817650 hasRelatedWork W4247809087 @default.
• W4310817650 isParatext "false" @default.
• W4310817650 isRetracted "false" @default.
• W4310817650 workType "article" @default.