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- W4310938549 abstract "Fluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to new single-photon (SP) array detectors, which give access to an entirely new set of single-photon information. Together with the blooming of new SP LSM techniques and the development of tailored SP array detectors, there is a growing need for (i) DAQ systems capable of handling the high-throughput and high-resolution photon information generated by these detectors, and (ii) incorporating these DAQ protocols in existing fluorescence LSMs. We developed an open-source, low-cost, multi-channel time-tagging module (TTM) based on a field-programmable gate array that can tag in parallel multiple single-photon events, with 30 ps precision, and multiple synchronisation events, with 4 ns precision. We use the TTM to demonstrate live-cell super-resolved fluorescence lifetime image scanning microscopy and fluorescence lifetime fluctuation spectroscopy. We expect that our BrightEyes-TTM will support the microscopy community in spreading SP-LSM in many life science laboratories." @default.
- W4310938549 created "2022-12-21" @default.
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- W4310938549 date "2022-12-01" @default.
- W4310938549 modified "2023-10-01" @default.
- W4310938549 title "The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy" @default.
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- W4310938549 doi "https://doi.org/10.1038/s41467-022-35064-0" @default.
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