FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4310940015> ?p ?o ?g. }

• W4310940015 endingPage "1033" @default.
• W4310940015 startingPage "1019" @default.
• W4310940015 abstract "Nucleotide excision repair (NER) is critical for removing bulky DNA base lesions and avoiding diseases. NER couples lesion recognition by XPC to strand separation by XPB and XPD ATPases, followed by lesion excision by XPF and XPG nucleases. Here, we describe key regulatory mechanisms and roles of XPG for and beyond its cleavage activity. Strikingly, by combing single-molecule imaging and bulk cleavage assays, we found that XPG binding to the 7-subunit TFIIH core (coreTFIIH) stimulates coreTFIIH-dependent double-strand (ds)DNA unwinding 10-fold, and XPG-dependent DNA cleavage by up to 700-fold. Simultaneous monitoring of rates for coreTFIIH single-stranded (ss)DNA translocation and dsDNA unwinding showed XPG acts by switching ssDNA translocation to dsDNA unwinding as a likely committed step. Pertinent to the NER pathway regulation, XPG incision activity is suppressed during coreTFIIH translocation on DNA but is licensed when coreTFIIH stalls at the lesion or when ATP hydrolysis is blocked. Moreover, ≥15 nucleotides of 5'-ssDNA is a prerequisite for efficient translocation and incision. Our results unveil a paired coordination mechanism in which key lesion scanning and DNA incision steps are sequentially coordinated, and damaged patch removal is only licensed after generation of ≥15 nucleotides of 5'-ssDNA, ensuring the correct ssDNA bubble size before cleavage.Nucleotide excision repair (NER) removes bulky DNA lesions and is thereby crucial in maintaining transcription and genomic integrity. Here, the authors show a dual function for the XPG nuclease that is critical for finding and excising the damage. During the separation of the damage-containing strand from the undamaged strand, XPG stimulates TFIIH dependent dsDNA unwinding 10 fold. In return, when TFIIH stalls at the damage it stimulates XPG nuclease activity 700 fold. Remarkably, this mutually exclusive coordination requires a bubble longer than 15 nucleotides. This study addressees why a bubble of a certain size is needed to facilitate NER and why XPG is recruited at the beginning of NER when its endonucleolytic activity is required at the very end." @default.
• W4310940015 created "2022-12-21" @default.
• W4310940015 creator A5000463966 @default.
• W4310940015 creator A5006718110 @default.
• W4310940015 creator A5036415725 @default.
• W4310940015 creator A5053835501 @default.
• W4310940015 creator A5056768778 @default.
• W4310940015 creator A5059713279 @default.
• W4310940015 creator A5068509466 @default.
• W4310940015 creator A5073290089 @default.
• W4310940015 creator A5079924550 @default.
• W4310940015 creator A5080832631 @default.
• W4310940015 creator A5087405697 @default.
• W4310940015 creator A5089057703 @default.
• W4310940015 date "2022-12-08" @default.
• W4310940015 modified "2023-10-06" @default.
• W4310940015 title "A scanning-to-incision switch in TFIIH-XPG induced by DNA damage licenses nucleotide excision repair" @default.
• W4310940015 cites W1485062679 @default.
• W4310940015 cites W1508748054 @default.
• W4310940015 cites W1516180781 @default.
• W4310940015 cites W1886853229 @default.
• W4310940015 cites W1942110646 @default.
• W4310940015 cites W1971439989 @default.
• W4310940015 cites W1974691766 @default.
• W4310940015 cites W1975365355 @default.
• W4310940015 cites W1977475171 @default.
• W4310940015 cites W1990849368 @default.
• W4310940015 cites W1993861248 @default.
• W4310940015 cites W1998495506 @default.
• W4310940015 cites W2002484799 @default.
• W4310940015 cites W2004640582 @default.
• W4310940015 cites W2008162481 @default.
• W4310940015 cites W2012085970 @default.
• W4310940015 cites W2038060623 @default.
• W4310940015 cites W2040150820 @default.
• W4310940015 cites W2044000184 @default.
• W4310940015 cites W2045155739 @default.
• W4310940015 cites W2046727880 @default.
• W4310940015 cites W2054693978 @default.
• W4310940015 cites W2056556017 @default.
• W4310940015 cites W2057663036 @default.
• W4310940015 cites W2057692066 @default.
• W4310940015 cites W2062028908 @default.
• W4310940015 cites W2067167274 @default.
• W4310940015 cites W2080605611 @default.
• W4310940015 cites W2082139407 @default.
• W4310940015 cites W2082749639 @default.
• W4310940015 cites W2084744430 @default.
• W4310940015 cites W2085980948 @default.
• W4310940015 cites W2088703460 @default.
• W4310940015 cites W2089788910 @default.
• W4310940015 cites W2093400437 @default.
• W4310940015 cites W2097124161 @default.
• W4310940015 cites W2098711782 @default.
• W4310940015 cites W2121909403 @default.
• W4310940015 cites W2127678435 @default.
• W4310940015 cites W2128552081 @default.
• W4310940015 cites W2129599566 @default.
• W4310940015 cites W2130027970 @default.
• W4310940015 cites W2131453199 @default.
• W4310940015 cites W2142587778 @default.
• W4310940015 cites W2142644202 @default.
• W4310940015 cites W2154261378 @default.
• W4310940015 cites W2156324690 @default.
• W4310940015 cites W2172916891 @default.
• W4310940015 cites W2589787895 @default.
• W4310940015 cites W2616493357 @default.
• W4310940015 cites W2619219946 @default.
• W4310940015 cites W2728852806 @default.
• W4310940015 cites W2782540500 @default.
• W4310940015 cites W2793725693 @default.
• W4310940015 cites W2793734738 @default.
• W4310940015 cites W2801246231 @default.
• W4310940015 cites W2953821629 @default.
• W4310940015 cites W2974077203 @default.
• W4310940015 cites W2990711314 @default.
• W4310940015 cites W3033172925 @default.
• W4310940015 cites W3087683486 @default.
• W4310940015 cites W3103614587 @default.
• W4310940015 cites W3104895458 @default.
• W4310940015 cites W3155078366 @default.
• W4310940015 cites W3168239620 @default.
• W4310940015 cites W3177828909 @default.
• W4310940015 cites W3190653485 @default.
• W4310940015 cites W3211795435 @default.
• W4310940015 cites W4210767609 @default.
• W4310940015 cites W4283067856 @default.
• W4310940015 cites W4291652370 @default.
• W4310940015 doi "https://doi.org/10.1093/nar/gkac1095" @default.
• W4310940015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36477609" @default.
• W4310940015 hasPublicationYear "2022" @default.
• W4310940015 type Work @default.
• W4310940015 citedByCount "4" @default.
• W4310940015 countsByYear W43109400152023 @default.
• W4310940015 crossrefType "journal-article" @default.
• W4310940015 hasAuthorship W4310940015A5000463966 @default.
• W4310940015 hasAuthorship W4310940015A5006718110 @default.
• W4310940015 hasAuthorship W4310940015A5036415725 @default.

• next