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- W4310952016 abstract "Abstract Background There is no standardized storage temperature of whole blood for acute normovolemic hemodilution (ANH). Study Design and Methods We conducted a prospective observational study to examine the difference in platelet function between short‐term whole blood storage at 4 and 22°C. Venous blood (40 ml) was collected from seven healthy subjects who gave prior written consent. The samples were divided into three groups: before storage (group Pre), cold (4°C) storage (group C), and room temperature (22°C) storage (group R). Groups C and R were tested after 6 h of blood storage. Platelet aggregability, platelet factor 4 (PF4), β ‐thromboglobulin ( β ‐TG), P‐selectin expression, pH, PO 2 , PCO 2 , glucose, lactate, blood count, and thromboelastography (TEG) parameters were measured. The percentage change in each parameter in groups C and R was calculated using the value in group Pre as a reference. These data were then compared between groups C and R using a Wilcoxon matched pairs test. p < 0.05 was considered to be statistically significant. Results Compared with group R, group C showed significantly higher platelet aggregability with adenosine diphosphate (ADP) 2, 4, and 6 μM (all p = 0.016) and collagen 1 μg/ml ( p = 0.047) stimulation, and significantly lower PF4 and β ‐TG elevation (both p = 0.031), glucose consumption ( p = 0.031), and lactate production ( p = 0.016). The ADP channel in TEG showed a significant increase in platelet aggregation rate in group C compared to group R. Discussion Cold storage of whole blood in ANH may provide improved storage conditions for platelets and contribute to improved hemostasis compared to room temperature storage." @default.
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- W4310952016 date "2022-12-07" @default.
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- W4310952016 title "Platelet function of whole blood after short‐term cold storage: A prospective in vitro observational study" @default.
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- W4310952016 doi "https://doi.org/10.1111/trf.17216" @default.
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