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• W4311049246 abstract "The effect of space flight factors and the subsequent adaptation to the Earth's gravity on oocytes is still poorly understood. Studies of mammalian oocytes in space present significant technical difficulties; therefore, the fruit fly Drosophila melanogaster is a convenient test subject. In this study, we analyzed the structure of the oocytes of the fruit fly Drosophila melanogaster, the maturation of which took place under space flight conditions (the Cytomehanarium experiment on the Russian Segment of the ISS during the ISS-67 expedition). The collection of the oocytes began immediately after landing and continued for 12 h. The flies were then transferred onto fresh agar plates and oocyte collection continued for the subsequent 12 h. The stiffness of oocytes was determined by atomic force microscopy and the content of the cytoskeletal proteins by Western blotting. The results demonstrated a significant decrease in the stiffness of oocytes in the flight group compared to the control (26.5 ± 1.1 pN/nm vs. 31.0 ± 1.8 pN/nm) against the background of a decrease in the content of some cytoskeletal proteins involved in the formation of microtubules and microfilaments. This pattern of oocyte structure leads to the disruption of cytokinesis during the cleavage of early embryos." @default.
• W4311049246 created "2022-12-23" @default.
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• W4311049246 date "2022-12-01" @default.
• W4311049246 modified "2023-10-18" @default.
• W4311049246 title "Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity" @default.
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• W4311049246 doi "https://doi.org/10.3390/cells11233871" @default.
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