Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311098412> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W4311098412 endingPage "B001" @default.
- W4311098412 startingPage "B001" @default.
- W4311098412 abstract "Abstract Epigenetic cancers lead to tumor progression via oncogene activation, tumor suppressor silencing, cell fate transitions, and genomic instability. Approximately 50% of tumors have alterations in Chromatin regulators. One group of Chromatin regulators, the mSWI/SNF complexes, are mutated in 20% of tumors making them one of the most mutated chromatin regulator complexes in cancer. In the past, the mechanism of action of ATP-dependent chromatin regulators, such as mSWI/SNF was largely explored using in vitro methods based on measurements of nucleosome mobility. These studies were limited by the fact that these assays were not sensitive to tissue-specific chromatin modifications, topology, long-range interactions, and complex patterns of histone modifications as well as patterns of DNA methylation and human disease mutational backgrounds. To circumvent these problems, we developed the FIRE-Cas9 system that allows one to recruit a specific chromatin regulator and follow the consequences with minute-by-minute kinetics on physiologic chromatin. I can examine any locus of biological or medical importance in virtually any cell type using this technique. We can recruit endogenous proteins or protein complexes such as different mSWI/SNF complexes, histone methyltransferases, or histone demethylases and assay changes in chromatin state and transcriptional state. Because chromatin regulator complexes have alterations in many human cancers, it is imperative to understand the mechanism of action of these complexes with unparalleled precision as a basis for therapeutic development. The FIRE-Cas9 system allows one to do this for the first time. Citation Format: Mary Bergwell, JinYoung Park, Laura Pistoni, Jacob G. Kirkland. Chromatin engineering using a dCas9-based inducible dimerization system. [abstract]. In: Proceedings of the AACR Special Conference: Cancer Epigenomics; 2022 Oct 6-8; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2022;82(23 Suppl_2):Abstract nr B001." @default.
- W4311098412 created "2022-12-23" @default.
- W4311098412 creator A5040730065 @default.
- W4311098412 creator A5068799024 @default.
- W4311098412 creator A5075577310 @default.
- W4311098412 creator A5078163455 @default.
- W4311098412 date "2022-12-01" @default.
- W4311098412 modified "2023-10-18" @default.
- W4311098412 title "Abstract B001: Chromatin engineering using a dCas9-based inducible dimerization system" @default.
- W4311098412 doi "https://doi.org/10.1158/1538-7445.cancepi22-b001" @default.
- W4311098412 hasPublicationYear "2022" @default.
- W4311098412 type Work @default.
- W4311098412 citedByCount "0" @default.
- W4311098412 crossrefType "journal-article" @default.
- W4311098412 hasAuthorship W4311098412A5040730065 @default.
- W4311098412 hasAuthorship W4311098412A5068799024 @default.
- W4311098412 hasAuthorship W4311098412A5075577310 @default.
- W4311098412 hasAuthorship W4311098412A5078163455 @default.
- W4311098412 hasConcept C104317684 @default.
- W4311098412 hasConcept C137338518 @default.
- W4311098412 hasConcept C167227067 @default.
- W4311098412 hasConcept C41091548 @default.
- W4311098412 hasConcept C502942594 @default.
- W4311098412 hasConcept C54355233 @default.
- W4311098412 hasConcept C552990157 @default.
- W4311098412 hasConcept C56952853 @default.
- W4311098412 hasConcept C64927066 @default.
- W4311098412 hasConcept C6929976 @default.
- W4311098412 hasConcept C83640560 @default.
- W4311098412 hasConcept C86803240 @default.
- W4311098412 hasConcept C95444343 @default.
- W4311098412 hasConceptScore W4311098412C104317684 @default.
- W4311098412 hasConceptScore W4311098412C137338518 @default.
- W4311098412 hasConceptScore W4311098412C167227067 @default.
- W4311098412 hasConceptScore W4311098412C41091548 @default.
- W4311098412 hasConceptScore W4311098412C502942594 @default.
- W4311098412 hasConceptScore W4311098412C54355233 @default.
- W4311098412 hasConceptScore W4311098412C552990157 @default.
- W4311098412 hasConceptScore W4311098412C56952853 @default.
- W4311098412 hasConceptScore W4311098412C64927066 @default.
- W4311098412 hasConceptScore W4311098412C6929976 @default.
- W4311098412 hasConceptScore W4311098412C83640560 @default.
- W4311098412 hasConceptScore W4311098412C86803240 @default.
- W4311098412 hasConceptScore W4311098412C95444343 @default.
- W4311098412 hasIssue "23_Supplement_2" @default.
- W4311098412 hasLocation W43110984121 @default.
- W4311098412 hasOpenAccess W4311098412 @default.
- W4311098412 hasPrimaryLocation W43110984121 @default.
- W4311098412 hasRelatedWork W1975603630 @default.
- W4311098412 hasRelatedWork W1998365877 @default.
- W4311098412 hasRelatedWork W2033355462 @default.
- W4311098412 hasRelatedWork W2056093527 @default.
- W4311098412 hasRelatedWork W2084943681 @default.
- W4311098412 hasRelatedWork W2156301924 @default.
- W4311098412 hasRelatedWork W2801503232 @default.
- W4311098412 hasRelatedWork W2986426317 @default.
- W4311098412 hasRelatedWork W3086059330 @default.
- W4311098412 hasRelatedWork W4224311492 @default.
- W4311098412 hasVolume "82" @default.
- W4311098412 isParatext "false" @default.
- W4311098412 isRetracted "false" @default.
- W4311098412 workType "article" @default.