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• W4311157444 abstract "Clostridioides difficile infection (CDI) is the most common cause of healthcare-associated diarrhoea in western countries, being categorized as an urgent healthcare threat. Historically, researchers have relied on the use of in vivo animal models to study CDI pathogenesis; however, differences in physiology and disease prognosis compared with humans limit their suitability to model CDI. In vitro models are increasingly being used as an alternative as they offer excellent process control, and some are able to use human ex-vivo prokaryotic and/or eukaryotic cells.Simulating the colonic environment in vitro is particularly challenging. Bacterial fermentation models have been used to evaluate novel therapeutics, explore the re-modelling of the gut microbiota, and simulate disease progression. However, they lack the scalability to become more widespread. Models that co-culture human and bacterial cells are of particular interest, but the different conditions required by each cell type make these models challenging to run. Recent advancements in model design have allowed for longer culture times with more representative bacterial populations.As in vitro models continue to evolve, they become more physiologically relevant, offering improved simulations of CDI, and extending their applicability." @default.
• W4311157444 created "2022-12-24" @default.
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• W4311157444 date "2022-11-03" @default.
• W4311157444 modified "2023-09-26" @default.
• W4311157444 title "In vitro models to study Clostridioides difficile infection: current systems and future advances" @default.
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• W4311157444 doi "https://doi.org/10.1097/mog.0000000000000893" @default.
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