Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311168514> ?p ?o ?g. }
- W4311168514 abstract "Antibodies are essential biological research tools and important therapeutic agents, but some exhibit non-specific binding to off-target proteins and other biomolecules. Such polyreactive antibodies compromise screening pipelines, lead to incorrect and irreproducible experimental results, and are generally intractable for clinical development. Here, we design a set of experiments using a diverse naïve synthetic camelid antibody fragment (nanobody) library to enable machine learning models to accurately assess polyreactivity from protein sequence (AUC > 0.8). Moreover, our models provide quantitative scoring metrics that predict the effect of amino acid substitutions on polyreactivity. We experimentally test our models' performance on three independent nanobody scaffolds, where over 90% of predicted substitutions successfully reduced polyreactivity. Importantly, the models allow us to diminish the polyreactivity of an angiotensin II type I receptor antagonist nanobody, without compromising its functional properties. We provide a companion web-server that offers a straightforward means of predicting polyreactivity and polyreactivity-reducing mutations for any given nanobody sequence." @default.
- W4311168514 created "2022-12-24" @default.
- W4311168514 creator A5000435697 @default.
- W4311168514 creator A5011428428 @default.
- W4311168514 creator A5011558149 @default.
- W4311168514 creator A5013891106 @default.
- W4311168514 creator A5035752038 @default.
- W4311168514 creator A5042213939 @default.
- W4311168514 creator A5044060561 @default.
- W4311168514 creator A5047333459 @default.
- W4311168514 creator A5050303147 @default.
- W4311168514 creator A5054642358 @default.
- W4311168514 creator A5071808580 @default.
- W4311168514 creator A5077979581 @default.
- W4311168514 date "2022-12-07" @default.
- W4311168514 modified "2023-10-12" @default.
- W4311168514 title "An in silico method to assess antibody fragment polyreactivity" @default.
- W4311168514 cites W1592986776 @default.
- W4311168514 cites W1652624883 @default.
- W4311168514 cites W1855237549 @default.
- W4311168514 cites W1975304761 @default.
- W4311168514 cites W1978090240 @default.
- W4311168514 cites W1981981290 @default.
- W4311168514 cites W1983335488 @default.
- W4311168514 cites W1984707191 @default.
- W4311168514 cites W1994133027 @default.
- W4311168514 cites W2018717876 @default.
- W4311168514 cites W2021160162 @default.
- W4311168514 cites W2038591876 @default.
- W4311168514 cites W2052844324 @default.
- W4311168514 cites W2076198413 @default.
- W4311168514 cites W2113904137 @default.
- W4311168514 cites W2122225224 @default.
- W4311168514 cites W2137216168 @default.
- W4311168514 cites W2144234665 @default.
- W4311168514 cites W2151125872 @default.
- W4311168514 cites W2156201868 @default.
- W4311168514 cites W2159047076 @default.
- W4311168514 cites W2163341755 @default.
- W4311168514 cites W2167745909 @default.
- W4311168514 cites W2170179543 @default.
- W4311168514 cites W2173513233 @default.
- W4311168514 cites W2487118445 @default.
- W4311168514 cites W2578029714 @default.
- W4311168514 cites W2594675420 @default.
- W4311168514 cites W2737510383 @default.
- W4311168514 cites W2739427485 @default.
- W4311168514 cites W2745083409 @default.
- W4311168514 cites W2756462780 @default.
- W4311168514 cites W2768658827 @default.
- W4311168514 cites W2786832567 @default.
- W4311168514 cites W2791362882 @default.
- W4311168514 cites W2806347806 @default.
- W4311168514 cites W2909414856 @default.
- W4311168514 cites W2910737538 @default.
- W4311168514 cites W2910849968 @default.
- W4311168514 cites W2914081542 @default.
- W4311168514 cites W2949064374 @default.
- W4311168514 cites W2955748570 @default.
- W4311168514 cites W2975542828 @default.
- W4311168514 cites W2983988502 @default.
- W4311168514 cites W3030808598 @default.
- W4311168514 cites W3047014325 @default.
- W4311168514 cites W3096751000 @default.
- W4311168514 cites W3106152681 @default.
- W4311168514 cites W3135072281 @default.
- W4311168514 cites W3139404958 @default.
- W4311168514 cites W3173700036 @default.
- W4311168514 cites W3176264499 @default.
- W4311168514 cites W3213415562 @default.
- W4311168514 cites W3217314753 @default.
- W4311168514 cites W4220765272 @default.
- W4311168514 cites W4221053240 @default.
- W4311168514 cites W4248872320 @default.
- W4311168514 cites W4283744078 @default.
- W4311168514 cites W4285039432 @default.
- W4311168514 doi "https://doi.org/10.1038/s41467-022-35276-4" @default.
- W4311168514 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36477674" @default.
- W4311168514 hasPublicationYear "2022" @default.
- W4311168514 type Work @default.
- W4311168514 citedByCount "6" @default.
- W4311168514 countsByYear W43111685142023 @default.
- W4311168514 crossrefType "journal-article" @default.
- W4311168514 hasAuthorship W4311168514A5000435697 @default.
- W4311168514 hasAuthorship W4311168514A5011428428 @default.
- W4311168514 hasAuthorship W4311168514A5011558149 @default.
- W4311168514 hasAuthorship W4311168514A5013891106 @default.
- W4311168514 hasAuthorship W4311168514A5035752038 @default.
- W4311168514 hasAuthorship W4311168514A5042213939 @default.
- W4311168514 hasAuthorship W4311168514A5044060561 @default.
- W4311168514 hasAuthorship W4311168514A5047333459 @default.
- W4311168514 hasAuthorship W4311168514A5050303147 @default.
- W4311168514 hasAuthorship W4311168514A5054642358 @default.
- W4311168514 hasAuthorship W4311168514A5071808580 @default.
- W4311168514 hasAuthorship W4311168514A5077979581 @default.
- W4311168514 hasBestOaLocation W43111685141 @default.
- W4311168514 hasConcept C104317684 @default.
- W4311168514 hasConcept C159654299 @default.
- W4311168514 hasConcept C185592680 @default.
- W4311168514 hasConcept C2775905019 @default.