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- W4311197382 abstract "ABSTRACT Enhancers function with a basal promoter to control the transcription of target genes. Enhancer regulatory activity is often studied using reporter-based transgene assays. However, unmatched results have been reported when selected enhancers are silenced in situ . In this study, using genomic deletion analysis in mice, we investigated the roles of two previously identified enhancers and the promoter of the Rho gene that codes for the visual pigment rhodopsin. The Rho gene is robustly expressed by rod photoreceptors of the retina, and essential for the subcellular structure and visual function of rod photoreceptors. Mutations in RHO cause severe vision loss in humans. We found that each Rho regulatory region can independently mediate local epigenomic changes, but only the promoter is absolutely required for establishing active Rho chromatin configuration and transcription and maintaining the cell integrity and function of rod photoreceptors. To our surprise, two Rho enhancers that enable strong promoter activation in reporter assays are largely dispensable for Rho expression in vivo . Only small and age-dependent impact is detectable when both enhancers are deleted. Our results demonstrate context-dependent roles of enhancers and highlight the importance of studying functions of cis - regulatory regions in the native genomic context." @default.
- W4311197382 created "2022-12-24" @default.
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- W4311197382 date "2022-12-02" @default.
- W4311197382 modified "2023-09-27" @default.
- W4311197382 title "<i>Rho</i>enhancers play unexpectedly minor roles in<i>Rhodopsin</i>transcription and rod cell integrity" @default.
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- W4311197382 doi "https://doi.org/10.1101/2022.12.02.518920" @default.
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