Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311303105> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W4311303105 endingPage "108766" @default.
- W4311303105 startingPage "108766" @default.
- W4311303105 abstract "L-threo-DOPS (L-threo-3,4-dihydroxyphenylserine) is a powerful anti-Parkinson's disease drug approved by FDA in 2014. The industrial production of optical L-threo-DOPS relied on optical resolution after chemical reactions, which did not fit into the atom-economy principle. L-Threonine aldolase (L-TA) could catalyze the synthesis of L-threo-DOPS from glycine and 3,4-dihydroxybenzaldehyde in one step, which is a promising alternative route. However, the substrate 3,4-dihydroxybenzaldehyde bearing an electron-rich catechol group is inactive for aldol condensation reaction, resulting in poor Cβ diastereoselectivity and low yields in enzymatic synthesis. In this work, virtual protein mutagenesis revealed the importance of His128 of L-TA to its binding with L-threo-DOPS intermediate. Further, the site-saturation mutagenesis of His128 provided a high-diastereoselectivity mutant H128N with 92.9 % de in the synthesis of L-threo-DOPS. With the whole cells harboring H128N, L-threo-DOPS was synthesized in a great de value of 95.4 % and a yield of 1.6 g/L in 1 h, which was significantly superior to the best-ever enzyme (55.4 % de). Based on the mutagenesis result and in silico docking, it was suggested that the enlarged volume and active hydrogen donor of the side chain of the amino acid 128 were disadvantageous, while proper hydrogen receptors could enhance substrate binding, leading to improved conversion." @default.
- W4311303105 created "2022-12-25" @default.
- W4311303105 creator A5013576420 @default.
- W4311303105 creator A5018626623 @default.
- W4311303105 creator A5022588348 @default.
- W4311303105 creator A5022952181 @default.
- W4311303105 creator A5057489654 @default.
- W4311303105 creator A5068273934 @default.
- W4311303105 creator A5080404415 @default.
- W4311303105 date "2023-02-01" @default.
- W4311303105 modified "2023-09-25" @default.
- W4311303105 title "Improving the Cβ stereoselectivity of L-threonine aldolase for the preparation of L-threo-3,4-dihydroxyphenylserine, a powerful anti-Parkinson's disease drug" @default.
- W4311303105 cites W2018570983 @default.
- W4311303105 cites W2032213049 @default.
- W4311303105 cites W2068713888 @default.
- W4311303105 cites W2076798074 @default.
- W4311303105 cites W2149525061 @default.
- W4311303105 cites W2260619470 @default.
- W4311303105 cites W2513486036 @default.
- W4311303105 cites W2696101647 @default.
- W4311303105 cites W2749178201 @default.
- W4311303105 cites W2911677253 @default.
- W4311303105 cites W2940330501 @default.
- W4311303105 cites W2967405228 @default.
- W4311303105 cites W3045909863 @default.
- W4311303105 cites W3102442198 @default.
- W4311303105 cites W3134922239 @default.
- W4311303105 cites W3156483590 @default.
- W4311303105 doi "https://doi.org/10.1016/j.bej.2022.108766" @default.
- W4311303105 hasPublicationYear "2023" @default.
- W4311303105 type Work @default.
- W4311303105 citedByCount "0" @default.
- W4311303105 crossrefType "journal-article" @default.
- W4311303105 hasAuthorship W4311303105A5013576420 @default.
- W4311303105 hasAuthorship W4311303105A5018626623 @default.
- W4311303105 hasAuthorship W4311303105A5022588348 @default.
- W4311303105 hasAuthorship W4311303105A5022952181 @default.
- W4311303105 hasAuthorship W4311303105A5057489654 @default.
- W4311303105 hasAuthorship W4311303105A5068273934 @default.
- W4311303105 hasAuthorship W4311303105A5080404415 @default.
- W4311303105 hasConcept C104317684 @default.
- W4311303105 hasConcept C109129352 @default.
- W4311303105 hasConcept C143065580 @default.
- W4311303105 hasConcept C161790260 @default.
- W4311303105 hasConcept C16318435 @default.
- W4311303105 hasConcept C181199279 @default.
- W4311303105 hasConcept C181647583 @default.
- W4311303105 hasConcept C185592680 @default.
- W4311303105 hasConcept C18903297 @default.
- W4311303105 hasConcept C2777289219 @default.
- W4311303105 hasConcept C28165981 @default.
- W4311303105 hasConcept C55493867 @default.
- W4311303105 hasConcept C71240020 @default.
- W4311303105 hasConcept C86803240 @default.
- W4311303105 hasConceptScore W4311303105C104317684 @default.
- W4311303105 hasConceptScore W4311303105C109129352 @default.
- W4311303105 hasConceptScore W4311303105C143065580 @default.
- W4311303105 hasConceptScore W4311303105C161790260 @default.
- W4311303105 hasConceptScore W4311303105C16318435 @default.
- W4311303105 hasConceptScore W4311303105C181199279 @default.
- W4311303105 hasConceptScore W4311303105C181647583 @default.
- W4311303105 hasConceptScore W4311303105C185592680 @default.
- W4311303105 hasConceptScore W4311303105C18903297 @default.
- W4311303105 hasConceptScore W4311303105C2777289219 @default.
- W4311303105 hasConceptScore W4311303105C28165981 @default.
- W4311303105 hasConceptScore W4311303105C55493867 @default.
- W4311303105 hasConceptScore W4311303105C71240020 @default.
- W4311303105 hasConceptScore W4311303105C86803240 @default.
- W4311303105 hasLocation W43113031051 @default.
- W4311303105 hasOpenAccess W4311303105 @default.
- W4311303105 hasPrimaryLocation W43113031051 @default.
- W4311303105 hasRelatedWork W1987826897 @default.
- W4311303105 hasRelatedWork W2003690817 @default.
- W4311303105 hasRelatedWork W2020748792 @default.
- W4311303105 hasRelatedWork W2032947420 @default.
- W4311303105 hasRelatedWork W2050485954 @default.
- W4311303105 hasRelatedWork W2074542695 @default.
- W4311303105 hasRelatedWork W2317084518 @default.
- W4311303105 hasRelatedWork W2561974534 @default.
- W4311303105 hasRelatedWork W2792698313 @default.
- W4311303105 hasRelatedWork W2945473828 @default.
- W4311303105 hasVolume "191" @default.
- W4311303105 isParatext "false" @default.
- W4311303105 isRetracted "false" @default.
- W4311303105 workType "article" @default.