FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311315098> ?p ?o ?g. }

• W4311315098 abstract "Abstract The aim of this study was to compare three different pretreatment protocols for the detection of 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) in nuclear DNA. Several types of human biological samples were analyzed, including formalin fixed, paraffin embedded (FFPE) normal squamous epithelium, ethanol fixed cultured cells and metaphase chromosomes. The antigen retrieval methods included the low pH citrate and high pH Tris/EDTA protocols, as well as a method including a pepsin pretreatment step combined with an HCl DNA denaturation step. We saw a gradual increase in the detection levels of 5-mC and 5-hmC when going from citrate via Tris/EDTA to pepsin/HCl retrieval. While the citrate retrieval protocol was the least efficient for the immunochemical detection of 5-mC and 5-hmC, it did preserve nuclear morphology and enabled the visualization of intra- and internuclear differences in FFPE tissue and cell culture samples. By simultaneous fluorescent detection, we assessed the differences in distribution patterns for both 5-mC and 5-hmC. In addition, (hydroxy)methylation levels in FFPE material were quantified using confocal and non-confocal microscopic imaging. As a result, we observed a significant heterogeneity, as well as differences between the levels for 5-mC and 5-hmC, within and between nuclei in the different compartments of normal squamous epithelium." @default.
• W4311315098 created "2022-12-25" @default.
• W4311315098 creator A5006975567 @default.
• W4311315098 creator A5046769792 @default.
• W4311315098 creator A5055215392 @default.
• W4311315098 creator A5081761895 @default.
• W4311315098 creator A5083290106 @default.
• W4311315098 date "2022-12-13" @default.
• W4311315098 modified "2023-10-15" @default.
• W4311315098 title "Immunohistochemical detection of global epigenetic DNA modifications using antibodies to 5-methylcytosine and 5-hydroxymethylcytosine. Impact of antigen retrieval protocols." @default.
• W4311315098 cites W1967184012 @default.
• W4311315098 cites W1969658866 @default.
• W4311315098 cites W1970145665 @default.
• W4311315098 cites W197255355 @default.
• W4311315098 cites W2013085398 @default.
• W4311315098 cites W2013346353 @default.
• W4311315098 cites W2029197798 @default.
• W4311315098 cites W2059520437 @default.
• W4311315098 cites W2077342495 @default.
• W4311315098 cites W2087752213 @default.
• W4311315098 cites W2092251734 @default.
• W4311315098 cites W2108664872 @default.
• W4311315098 cites W2111538587 @default.
• W4311315098 cites W2152636479 @default.
• W4311315098 cites W2152656267 @default.
• W4311315098 cites W2154668531 @default.
• W4311315098 cites W2167279371 @default.
• W4311315098 cites W2557768315 @default.
• W4311315098 cites W2887974597 @default.
• W4311315098 cites W2889121635 @default.
• W4311315098 cites W2954063797 @default.
• W4311315098 cites W3020424172 @default.
• W4311315098 cites W3095084877 @default.
• W4311315098 cites W3111292807 @default.
• W4311315098 cites W3138770880 @default.
• W4311315098 cites W3165106304 @default.
• W4311315098 cites W3168958685 @default.
• W4311315098 doi "https://doi.org/10.21203/rs.3.rs-2234522/v2" @default.
• W4311315098 hasPublicationYear "2022" @default.
• W4311315098 type Work @default.
• W4311315098 citedByCount "0" @default.
• W4311315098 crossrefType "posted-content" @default.
• W4311315098 hasAuthorship W4311315098A5006975567 @default.
• W4311315098 hasAuthorship W4311315098A5046769792 @default.
• W4311315098 hasAuthorship W4311315098A5055215392 @default.
• W4311315098 hasAuthorship W4311315098A5081761895 @default.
• W4311315098 hasAuthorship W4311315098A5083290106 @default.
• W4311315098 hasBestOaLocation W43113150981 @default.
• W4311315098 hasConcept C104317684 @default.
• W4311315098 hasConcept C115960442 @default.
• W4311315098 hasConcept C121332964 @default.
• W4311315098 hasConcept C136009344 @default.
• W4311315098 hasConcept C147483822 @default.
• W4311315098 hasConcept C150194340 @default.
• W4311315098 hasConcept C153911025 @default.
• W4311315098 hasConcept C159654299 @default.
• W4311315098 hasConcept C179687394 @default.
• W4311315098 hasConcept C185592680 @default.
• W4311315098 hasConcept C190727270 @default.
• W4311315098 hasConcept C24586158 @default.
• W4311315098 hasConcept C2524010 @default.
• W4311315098 hasConcept C2775926025 @default.
• W4311315098 hasConcept C2778901553 @default.
• W4311315098 hasConcept C2779484032 @default.
• W4311315098 hasConcept C33923547 @default.
• W4311315098 hasConcept C41091548 @default.
• W4311315098 hasConcept C54355233 @default.
• W4311315098 hasConcept C552990157 @default.
• W4311315098 hasConcept C55493867 @default.
• W4311315098 hasConcept C62520636 @default.
• W4311315098 hasConcept C86803240 @default.
• W4311315098 hasConcept C91881484 @default.
• W4311315098 hasConceptScore W4311315098C104317684 @default.
• W4311315098 hasConceptScore W4311315098C115960442 @default.
• W4311315098 hasConceptScore W4311315098C121332964 @default.
• W4311315098 hasConceptScore W4311315098C136009344 @default.
• W4311315098 hasConceptScore W4311315098C147483822 @default.
• W4311315098 hasConceptScore W4311315098C150194340 @default.
• W4311315098 hasConceptScore W4311315098C153911025 @default.
• W4311315098 hasConceptScore W4311315098C159654299 @default.
• W4311315098 hasConceptScore W4311315098C179687394 @default.
• W4311315098 hasConceptScore W4311315098C185592680 @default.
• W4311315098 hasConceptScore W4311315098C190727270 @default.
• W4311315098 hasConceptScore W4311315098C24586158 @default.
• W4311315098 hasConceptScore W4311315098C2524010 @default.
• W4311315098 hasConceptScore W4311315098C2775926025 @default.
• W4311315098 hasConceptScore W4311315098C2778901553 @default.
• W4311315098 hasConceptScore W4311315098C2779484032 @default.
• W4311315098 hasConceptScore W4311315098C33923547 @default.
• W4311315098 hasConceptScore W4311315098C41091548 @default.
• W4311315098 hasConceptScore W4311315098C54355233 @default.
• W4311315098 hasConceptScore W4311315098C552990157 @default.
• W4311315098 hasConceptScore W4311315098C55493867 @default.
• W4311315098 hasConceptScore W4311315098C62520636 @default.
• W4311315098 hasConceptScore W4311315098C86803240 @default.
• W4311315098 hasConceptScore W4311315098C91881484 @default.
• W4311315098 hasLocation W43113150981 @default.
• W4311315098 hasOpenAccess W4311315098 @default.
• W4311315098 hasPrimaryLocation W43113150981 @default.
• W4311315098 hasRelatedWork W2040183742 @default.

• next