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• W4311325394 abstract "Abstract Background Salmonella enterica , serovar Enteritidis (SE) is a food-borne pathogen, which can cause great threat to human health through consumption of the contaminated poultry products. Chicken is the main host of SE. The mRNA and microRNA (miRNA) expression profiles were analyzed on cecum of Shouguang chicken via next-generation sequencing and bioinformatics approaches. The treated group was inoculated SE, and the control group was inoculated with phosphate buffer saline (PBS). Results There were 1760 differentially expressed mRNAs in the SE-infected group, of which 1046 were up-regulated mRNA, and 714 were down-regulated mRNA. In addition, a total of 821 miRNAs were identified, and 174 miRNAs were differentially expressed, of which 100 were up-regulated and 74 were down-regulated. Functional enrichment of differentially expressed mRNAs was similar to miRNA target genes. The functional analysis results of differentially expressed mRNAs and miRNAs were performed. Immune-related processes and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways were enriched by up-regulated mRNA. The down-regulated mRNAs were enriched in tissue development and metabolic-related KEGG pathways. The functional analysis of up-regulated miRNA target genes was similar to the down-regulated mRNAs. The down-regulated miRNA target genes were enriched in metabolic-related GO (Gene Ontology) -BP (Biological process) terms and KEGG pathways. The overlap of the up-regulated mRNA and the up-regulated miRNA target genes (class I) was 325, and the overlap of the down-regulated miRNA target genes (class II) was 169. The class I enriched in the immune-related GO-BP terms and KEGG pathways. The class II mainly enriched in metabolic-related GO-BP terms and KEGG pathways. Then we detected the expression of mRNA and miRNA through qRT-PCR. The results shown that the expression of HHIP , PGM1 , HTR2B , ITGB5 , RELN, SFRP1 , TCF7L2 , SCNN1A , NEK7, miR-20b-5p, miR-1662, miR-15a, miR-16-1-3p was significantly different between two groups. Dual-luciferase reporter assay was used to detect the relationship between miR-20b-5p and SCNN1A . The result indicated that miR-20b-5p regulate immune or metabolic responses after SE infection in Shouguang chickens by directly targeting SCNN1A . Conclusions The findings here contribute to the further analysis of the mechanism of mRNA and miRNA defense against SE infection, and provide a theoretical foundation for the molecular disease-resistant breeding of chickens." @default.
• W4311325394 created "2022-12-25" @default.
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• W4311325394 date "2022-12-14" @default.
• W4311325394 modified "2023-09-26" @default.
• W4311325394 title "Regulation of mRNA and miRNA in the response to Salmonella enterica serovar Enteritidis infection in chicken cecum" @default.
• W4311325394 cites W1970717064 @default.
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• W4311325394 cites W1975072242 @default.
• W4311325394 cites W1976496419 @default.
• W4311325394 cites W1983470819 @default.
• W4311325394 cites W2002764864 @default.
• W4311325394 cites W2013523306 @default.
• W4311325394 cites W2013868665 @default.
• W4311325394 cites W2024932219 @default.
• W4311325394 cites W2061829501 @default.
• W4311325394 cites W2062099498 @default.
• W4311325394 cites W2081421614 @default.
• W4311325394 cites W2086320673 @default.
• W4311325394 cites W2104901075 @default.
• W4311325394 cites W2117391818 @default.
• W4311325394 cites W2127722031 @default.
• W4311325394 cites W2128944211 @default.
• W4311325394 cites W2135782580 @default.
• W4311325394 cites W2138651252 @default.
• W4311325394 cites W2145297900 @default.
• W4311325394 cites W2151081606 @default.
• W4311325394 cites W2156246479 @default.
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• W4311325394 cites W2166208529 @default.
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• W4311325394 cites W2576093325 @default.
• W4311325394 cites W2587345915 @default.
• W4311325394 cites W2783967041 @default.
• W4311325394 cites W2784128628 @default.
• W4311325394 cites W2788230178 @default.
• W4311325394 cites W2801611906 @default.
• W4311325394 cites W2806514710 @default.
• W4311325394 cites W2806580017 @default.
• W4311325394 cites W2884679847 @default.
• W4311325394 cites W2896798787 @default.
• W4311325394 cites W2898811889 @default.
• W4311325394 cites W2898823598 @default.
• W4311325394 cites W2900205177 @default.
• W4311325394 cites W2903751680 @default.
• W4311325394 cites W2921427977 @default.
• W4311325394 cites W2936325242 @default.
• W4311325394 cites W2939900094 @default.
• W4311325394 cites W2969718750 @default.
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• W4311325394 cites W3039118932 @default.
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• W4311325394 cites W3093216561 @default.
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• W4311325394 cites W3162535811 @default.
• W4311325394 cites W3178201248 @default.
• W4311325394 cites W3180654723 @default.
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• W4311325394 doi "https://doi.org/10.1186/s12917-022-03522-y" @default.
• W4311325394 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36514049" @default.
• W4311325394 hasPublicationYear "2022" @default.
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