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• W4311343896 endingPage "463716" @default.
• W4311343896 startingPage "463716" @default.
• W4311343896 abstract "Plastic microchips possess the advantages of easy fabrication and low-cost, but their surface properties are frequently incompatible with electrophoretic separation without proper surface modification. Meanwhile, the separation microchannels on typical microchips are usually only a few centimeters long, the pressurized flow may significantly affect the electrophoretic separation if their inner diameters (id) are relatively larger (approximately > 50 μm), viscous separation medium is therefore required for efficient separation. Herein, a zwitterionic surfactant, N-hexadecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate (HDAPS), was used as a multifunctional additive to inhibit the analyte adsorption, improve the surface status, control Joule heating and modulate the resolution on cyclic olefin copolymer microchips with 80 μm id, 5 cm long separation microchannels, eliminating the necessity of viscous polymeric additives. The effectiveness of HDAPS was compared with an ionic polymeric additive, poly(diallydimethylammonium chloride). The streaming potential and electroosmotic flow measurements indicated an effective inhibition of the adsorption of rhodamine B and a stable negative surface charge with zwitterionic HDAPS. Using 15 mmol/L HDAPS, 40% (v/v) methanol, and 10 mmol/L boric acid (pH 3.2) as the running buffer, rapid separation of four rhodamines was achieved within 90 s under a separation electric field of 520 V/cm. The theoretical plate numbers were in a range of 5.0×105–6.9×105/m. The relative standard deviations were no more than 0.9% for retention time and 1.5% for peak area. The proposed system was verified by the determination of rhodamines in eyeshadow and wolfberry, with standard recoveries in a range of 98.2%–101.4%." @default.
• W4311343896 created "2022-12-25" @default.
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• W4311343896 date "2023-01-01" @default.
• W4311343896 modified "2023-09-27" @default.
• W4311343896 title "Zwitterionic surfactant as an additive for efficient electrophoretic separation of easily absorbed rhodamine dyes on plastic microchips" @default.
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• W4311343896 doi "https://doi.org/10.1016/j.chroma.2022.463716" @default.
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