Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311403451> ?p ?o ?g. }
- W4311403451 abstract "DNAzymes effectively inhibit the expression of viral genes. Duck hepatitis A virus type-1 (DHAV-1) genomic RNA carries an internal ribosome entry site (IRES). The IRES initiates the translation of DHAV-1 via a mechanism that differs from that of cap-dependent translation. Therefore, it is an attractive target for the treatment of DHAV-1. In this study, we designed 6 DNAzymes (Dzs) specifically targeting 300-618 nt sequence in the DHAV-1 5'untranslated region (UTR; a predicted IRES-like element). In the presence of divalent metal ions, three designed DNAzymes (DZ369, DZ454, and DZ514) efficiently cleaved the 300-618 nt sequence of the DHAV-1 5'UTR RNA. The activity of the Dzs was particularly dependent on Mg2+ ions. Subsequently, the translation inhibitory activity of these Dzs was determined by western blotting experiments. The Dzs effectively inhibited the translation mediated by the 300-618 nt of DHAV-1 5'UTR in duck embryo fibroblasts (DEFs). Importantly, DZ454 showed the strongest inhibitory effect, and its inhibition was time and dose dependent. However, none of the Dzs showed significant inhibition of cap-dependent translation. These results suggest that these Dzs show specificity for target RNA. Moreover, DZ454 inhibited the replication of DHAV-1. In conclusion, the designed DNAzymes can be used as inhibitors of DHAV-1 RNA translation and replication, providing new insights useful for the development of anti-DHAV-1 drugs." @default.
- W4311403451 created "2022-12-26" @default.
- W4311403451 creator A5000460901 @default.
- W4311403451 creator A5007648400 @default.
- W4311403451 creator A5007933016 @default.
- W4311403451 creator A5008178802 @default.
- W4311403451 creator A5010409371 @default.
- W4311403451 creator A5013454053 @default.
- W4311403451 creator A5019205025 @default.
- W4311403451 creator A5021517739 @default.
- W4311403451 creator A5028136131 @default.
- W4311403451 creator A5031331731 @default.
- W4311403451 creator A5035312767 @default.
- W4311403451 creator A5038302862 @default.
- W4311403451 creator A5039291898 @default.
- W4311403451 creator A5049672306 @default.
- W4311403451 creator A5064308879 @default.
- W4311403451 creator A5072909687 @default.
- W4311403451 creator A5075014478 @default.
- W4311403451 creator A5076996063 @default.
- W4311403451 creator A5083228843 @default.
- W4311403451 creator A5086086173 @default.
- W4311403451 creator A5088124858 @default.
- W4311403451 date "2022-12-12" @default.
- W4311403451 modified "2023-10-17" @default.
- W4311403451 title "Specific DNAzymes cleave the 300–618 nt of 5′UTR to inhibit DHAV-1 translation and replication" @default.
- W4311403451 cites W1807212217 @default.
- W4311403451 cites W1964053517 @default.
- W4311403451 cites W1968463586 @default.
- W4311403451 cites W1970617451 @default.
- W4311403451 cites W1971880211 @default.
- W4311403451 cites W1977709085 @default.
- W4311403451 cites W1981273992 @default.
- W4311403451 cites W1985904924 @default.
- W4311403451 cites W2001461169 @default.
- W4311403451 cites W2025502840 @default.
- W4311403451 cites W2030129667 @default.
- W4311403451 cites W2031662022 @default.
- W4311403451 cites W2060190614 @default.
- W4311403451 cites W2065862344 @default.
- W4311403451 cites W2073936523 @default.
- W4311403451 cites W2073982679 @default.
- W4311403451 cites W2097925217 @default.
- W4311403451 cites W2106769402 @default.
- W4311403451 cites W2110742125 @default.
- W4311403451 cites W2115470479 @default.
- W4311403451 cites W2124861729 @default.
- W4311403451 cites W2150742206 @default.
- W4311403451 cites W2167408428 @default.
- W4311403451 cites W2435532055 @default.
- W4311403451 cites W2608501046 @default.
- W4311403451 cites W2622743678 @default.
- W4311403451 cites W2766986299 @default.
- W4311403451 cites W2770378609 @default.
- W4311403451 cites W2771262230 @default.
- W4311403451 cites W2782474287 @default.
- W4311403451 cites W2888251530 @default.
- W4311403451 cites W2894151100 @default.
- W4311403451 cites W2901040100 @default.
- W4311403451 cites W2946894699 @default.
- W4311403451 cites W2987040874 @default.
- W4311403451 cites W3033932216 @default.
- W4311403451 cites W3036184198 @default.
- W4311403451 cites W3039230126 @default.
- W4311403451 cites W3080482538 @default.
- W4311403451 cites W3091223622 @default.
- W4311403451 cites W3137326065 @default.
- W4311403451 cites W3152494999 @default.
- W4311403451 cites W3155067013 @default.
- W4311403451 cites W3174750121 @default.
- W4311403451 cites W4200062047 @default.
- W4311403451 cites W4211212443 @default.
- W4311403451 cites W4232144512 @default.
- W4311403451 cites W4283588295 @default.
- W4311403451 cites W4292092977 @default.
- W4311403451 doi "https://doi.org/10.3389/fmicb.2022.1064612" @default.
- W4311403451 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36578574" @default.
- W4311403451 hasPublicationYear "2022" @default.
- W4311403451 type Work @default.
- W4311403451 citedByCount "1" @default.
- W4311403451 countsByYear W43114034512023 @default.
- W4311403451 crossrefType "journal-article" @default.
- W4311403451 hasAuthorship W4311403451A5000460901 @default.
- W4311403451 hasAuthorship W4311403451A5007648400 @default.
- W4311403451 hasAuthorship W4311403451A5007933016 @default.
- W4311403451 hasAuthorship W4311403451A5008178802 @default.
- W4311403451 hasAuthorship W4311403451A5010409371 @default.
- W4311403451 hasAuthorship W4311403451A5013454053 @default.
- W4311403451 hasAuthorship W4311403451A5019205025 @default.
- W4311403451 hasAuthorship W4311403451A5021517739 @default.
- W4311403451 hasAuthorship W4311403451A5028136131 @default.
- W4311403451 hasAuthorship W4311403451A5031331731 @default.
- W4311403451 hasAuthorship W4311403451A5035312767 @default.
- W4311403451 hasAuthorship W4311403451A5038302862 @default.
- W4311403451 hasAuthorship W4311403451A5039291898 @default.
- W4311403451 hasAuthorship W4311403451A5049672306 @default.
- W4311403451 hasAuthorship W4311403451A5064308879 @default.
- W4311403451 hasAuthorship W4311403451A5072909687 @default.
- W4311403451 hasAuthorship W4311403451A5075014478 @default.
- W4311403451 hasAuthorship W4311403451A5076996063 @default.