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- W4311436855 abstract "Purpose: Analysis of the first stage of monitoring the genetic variety of two genes of MTDNK of the mixed population of Nerca river. Ola. Materials and methods. Separate nonsense were caught during the spawning stroke at the mouth of the Ola river in late June - early July 2014 (13 individuals), 2020 (9 individuals) and 2021 (9 individuals). The material (muscles) was stored in a frozen state. DNA allocation and determination of nucleotide sequences of these genes are made in PJSC Sentol, Moscow. The sequences of the nucleotides of the studied MTDNK genes are leveled using the Clustal W program included in the MEGA version of version 11. The specified program was used to assess the value of nucleotide differences (R-disters) between individuals for the design of dendrograms. The clustering method for constructing dendrograms is unnecessary in pairs-group (UPGMA). The quantitative parameters of haplotypic and nucleotide diversity were found using the program DNASP, V. 5. Results. Nucleotide diversity of 31 sockeye salmon from three samples (2014, 2020 and 2021) from the population of the Ola River for the cytochrome oxidase gene of mtDNA subunit 1 (CO I) was the value π =0.00505±0.000080, haplotypic diversity Hd = 0.708±0.0042.These indicators for 18 individuals from two samples (2020 and 2021) for the cytochrome b gene (cyt b) mt DNA π = 0.00184± 0.000075 and Hd = 0.699±0.0158.Significant differences in the nucleotide sets of both genes between samples of different years were noted. It is possible that the detected interannual differences in the nucleotide sets of samples of sockeye salmon producers are associated with the influence of fish-breeding activities conducted by employees." @default.
- W4311436855 created "2022-12-26" @default.
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- W4311436855 date "2022-12-11" @default.
- W4311436855 modified "2023-09-25" @default.
- W4311436855 title "Genetic diversity of some mitochondrial DNA genes in a mixed population of sockeye salmon (<i>Oncorhynchus nerka</i>) of the Ola River (northern coast of the Sea of Okhotsk)" @default.
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- W4311436855 doi "https://doi.org/10.31043/2410-2733-2022-3-18-24" @default.
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