FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311456336> ?p ?o ?g. }

• W4311456336 endingPage "150" @default.
• W4311456336 startingPage "144" @default.
• W4311456336 abstract "Existing real-time reverse transcriptase PCR (RT-qPCR) has certain limitations for the point-of-care detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) since it requires sophisticated instruments, reagents and skilled laboratory personnel. In this study, we evaluated an assay termed the reverse transcriptase-polymerase spiral reaction (RT-PSR) for rapid and visual detection of SARS-CoV-2.The RT-PSR assay was optimized using RdRp gene and evaluated for the detection of SARS-CoV-2. The time of 60min and a temperature of 63°C was optimized for targeting the RNA-dependent RNA polymerase gene of SARS-CoV-2. The sensitivity of the assay was evaluated by diluting the in-vitro transcribed RNA, which amplifies as low as ten copies.The specific primers designed for this assay showed 100% specificity and did not react when tested with other lung infection-causing viruses and bacteria. The optimized assay was validated with 190 clinical samples in two phases, using automated RTPCR based TrueNat test, and the results were comparable.The RT-PSR assay can be considered for rapid and sensitive detection of SARS-CoV-2, particularly in resource-limited settings. To our knowledge, there is as yet no RT-PSR-based kit developed for SARS-CoV-2." @default.
• W4311456336 created "2022-12-26" @default.
• W4311456336 creator A5003373296 @default.
• W4311456336 creator A5021733468 @default.
• W4311456336 creator A5034840616 @default.
• W4311456336 creator A5040070856 @default.
• W4311456336 creator A5049998420 @default.
• W4311456336 creator A5050568235 @default.
• W4311456336 creator A5059484070 @default.
• W4311456336 creator A5077117975 @default.
• W4311456336 creator A5078874216 @default.
• W4311456336 creator A5088178160 @default.
• W4311456336 creator A5089393967 @default.
• W4311456336 date "2023-01-01" @default.
• W4311456336 modified "2023-09-26" @default.
• W4311456336 title "Evaluation of reverse transcriptase-polymerase spiral reaction assay for rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2" @default.
• W4311456336 cites W1975801865 @default.
• W4311456336 cites W2066385972 @default.
• W4311456336 cites W2104969756 @default.
• W4311456336 cites W2145303234 @default.
• W4311456336 cites W2404804741 @default.
• W4311456336 cites W2945040903 @default.
• W4311456336 cites W2982545901 @default.
• W4311456336 cites W3008294222 @default.
• W4311456336 cites W3010377921 @default.
• W4311456336 cites W3021274772 @default.
• W4311456336 cites W3022236459 @default.
• W4311456336 cites W3023897783 @default.
• W4311456336 cites W3024705579 @default.
• W4311456336 cites W3032292222 @default.
• W4311456336 cites W3045393582 @default.
• W4311456336 cites W3094064847 @default.
• W4311456336 cites W3094677842 @default.
• W4311456336 cites W3096541018 @default.
• W4311456336 cites W3102664441 @default.
• W4311456336 cites W3111460775 @default.
• W4311456336 cites W3119562705 @default.
• W4311456336 cites W3121036322 @default.
• W4311456336 cites W3182364916 @default.
• W4311456336 doi "https://doi.org/10.1016/j.cca.2022.12.009" @default.
• W4311456336 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36528050" @default.
• W4311456336 hasPublicationYear "2023" @default.
• W4311456336 type Work @default.
• W4311456336 citedByCount "1" @default.
• W4311456336 countsByYear W43114563362023 @default.
• W4311456336 crossrefType "journal-article" @default.
• W4311456336 hasAuthorship W4311456336A5003373296 @default.
• W4311456336 hasAuthorship W4311456336A5021733468 @default.
• W4311456336 hasAuthorship W4311456336A5034840616 @default.
• W4311456336 hasAuthorship W4311456336A5040070856 @default.
• W4311456336 hasAuthorship W4311456336A5049998420 @default.
• W4311456336 hasAuthorship W4311456336A5050568235 @default.
• W4311456336 hasAuthorship W4311456336A5059484070 @default.
• W4311456336 hasAuthorship W4311456336A5077117975 @default.
• W4311456336 hasAuthorship W4311456336A5078874216 @default.
• W4311456336 hasAuthorship W4311456336A5088178160 @default.
• W4311456336 hasAuthorship W4311456336A5089393967 @default.
• W4311456336 hasBestOaLocation W43114563361 @default.
• W4311456336 hasConcept C104317684 @default.
• W4311456336 hasConcept C105580179 @default.
• W4311456336 hasConcept C142724271 @default.
• W4311456336 hasConcept C153911025 @default.
• W4311456336 hasConcept C156719811 @default.
• W4311456336 hasConcept C159047783 @default.
• W4311456336 hasConcept C2777648638 @default.
• W4311456336 hasConcept C2779134260 @default.
• W4311456336 hasConcept C3008058167 @default.
• W4311456336 hasConcept C4692481 @default.
• W4311456336 hasConcept C48023723 @default.
• W4311456336 hasConcept C49105822 @default.
• W4311456336 hasConcept C524204448 @default.
• W4311456336 hasConcept C54355233 @default.
• W4311456336 hasConcept C67705224 @default.
• W4311456336 hasConcept C71924100 @default.
• W4311456336 hasConcept C82381507 @default.
• W4311456336 hasConcept C86803240 @default.
• W4311456336 hasConceptScore W4311456336C104317684 @default.
• W4311456336 hasConceptScore W4311456336C105580179 @default.
• W4311456336 hasConceptScore W4311456336C142724271 @default.
• W4311456336 hasConceptScore W4311456336C153911025 @default.
• W4311456336 hasConceptScore W4311456336C156719811 @default.
• W4311456336 hasConceptScore W4311456336C159047783 @default.
• W4311456336 hasConceptScore W4311456336C2777648638 @default.
• W4311456336 hasConceptScore W4311456336C2779134260 @default.
• W4311456336 hasConceptScore W4311456336C3008058167 @default.
• W4311456336 hasConceptScore W4311456336C4692481 @default.
• W4311456336 hasConceptScore W4311456336C48023723 @default.
• W4311456336 hasConceptScore W4311456336C49105822 @default.
• W4311456336 hasConceptScore W4311456336C524204448 @default.
• W4311456336 hasConceptScore W4311456336C54355233 @default.
• W4311456336 hasConceptScore W4311456336C67705224 @default.
• W4311456336 hasConceptScore W4311456336C71924100 @default.
• W4311456336 hasConceptScore W4311456336C82381507 @default.
• W4311456336 hasConceptScore W4311456336C86803240 @default.
• W4311456336 hasLocation W43114563361 @default.
• W4311456336 hasLocation W43114563362 @default.
• W4311456336 hasLocation W43114563363 @default.
• W4311456336 hasOpenAccess W4311456336 @default.

• next