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- W4311473226 abstract "Abstract Background: Rhizoctonia solani AG1 IA is an important pathogen of rice ( Oryza sativa L.) that causes rice sheath blight (RSB). Since control of RSB by conventional measures has failed, novel strategies like application of plant growth-promoting rhizobacteria (PGPR) can be an efficient alternative. Method and Results: mRNA sequences of rice were retrieving from NCBI for candidate reference genes selction, and seven candidate reference genes (RGs), namely 18SrRNA , ACT1 , GAPDH2 , UBC5 , RPS27 , eIF4a and CYP28, were selected for their stability in real-time quantitative PCR (RT-qPCR). Different algorithms were exploited, Delta Ct, geNorm, NormFinder, BestKeeper, and Comprehensive ranking by RefFinder, to evaluate RT-qPCR of rice in tissues infected with R. solani and treated with the PGPR strains, Pseudomonas saponiphilia and Pseudomonas protegens , and potassium silicate (KSi) alone or in combination with each PGPR strain. RGs stability was affected by each treatment and treatment-specific selection was approved and validated for nonexpressor of PR-1(NPR1) for each treatment. Conclusion : Overall, ACT1 was the most stable RG with R. solani infection alone, GAPDH2 with R. solani infection plus KSi, UBC5 with R. solani infection plus P. saponiphilia , and eIF4a with R. solani infection plus P. protegens . Both ACT1 and RPS27 were the most stable with the combination of KSi and P. saponiphilia , while PRS27 was the most stable with the combination of KSi and P. protegens" @default.
- W4311473226 created "2022-12-26" @default.
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- W4311473226 date "2022-12-14" @default.
- W4311473226 modified "2023-10-14" @default.
- W4311473226 title "Selection of reference genes for RT-qPCR analysis of rice with Rhizoctonia solani infection and PGPR/KSi application" @default.
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- W4311473226 doi "https://doi.org/10.21203/rs.3.rs-2362663/v1" @default.
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