FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311481014> ?p ?o ?g. }

• W4311481014 abstract "Clavulanic acid (CA) is the preferred clinical drug for the treatment of infections by β-lactam antibiotic-resistant bacteria. CA is produced by Streptomyces clavuligerus, and although there have been many reports on the effects of carbon and nitrogen sources on CA production, the mechanisms involved remain unclear. In this study, we found that CA accumulation in S. clavuligerus F613-1 was increased significantly in MH medium, which is rich in organic nitrogen, compared with that in ML medium, which contains half the amount of organic nitrogen present in MH medium. Transcriptome analysis revealed that genes involved in CA biosynthesis, such as ceas1, ceas2, bls1, bls2, cas2, pah2, gcaS, and cad, and arginine biosynthesis, such as argB, argC, argD, argG, argH, argJ, and argR, were upregulated under rich organic nitrogen. Metabolome data revealed notable differences between cultures of F613-1 grown in MH and ML media with regard to levels of key intracellular metabolites, most of which are involved in arginine metabolic pathways, including arginine, glutamine, and glutamic acid. Additionally, supplementation of ML medium with arginine, glutamine, or glutamic acid resulted in increased CA production by S. clavuligerus F613-1. Our results indicate that rich organic nitrogen mainly affects CA biosynthesis by increasing the levels of amino acids associated with the arginine metabolic pathway and activating the expression of the CA biosynthetic gene cluster. These findings provide important insights for improving medium optimization and engineering of S. clavuligerus F613-1 for high-yield production of CA. IMPORTANCE The bacterium Streptomyces clavuligerus is used for the industrial production of the broad-spectrum β-lactamase inhibitor clavulanic acid (CA). However, much remains unknown about the factors which affect CA yields. We investigated the effects of different levels of organic nitrogen on CA production. Our analyses indicate that higher organic nitrogen levels were associated with increased CA yields and increased levels of arginine biosynthesis. Further analyses supported the relationship between arginine metabolism and CA production and demonstrated that increasing the levels of arginine or associated amino acids could boost CA yields. These findings suggest approaches for improving the production of this clinically important antibiotic." @default.
• W4311481014 created "2022-12-26" @default.
• W4311481014 creator A5001577023 @default.
• W4311481014 creator A5024197542 @default.
• W4311481014 creator A5028468364 @default.
• W4311481014 creator A5047908354 @default.
• W4311481014 creator A5055008485 @default.
• W4311481014 creator A5078750267 @default.
• W4311481014 creator A5088055959 @default.
• W4311481014 date "2023-02-14" @default.
• W4311481014 modified "2023-09-30" @default.
• W4311481014 title "Rich Organic Nitrogen Impacts Clavulanic Acid Biosynthesis through the Arginine Metabolic Pathway in Streptomyces clavuligerus F613-1" @default.
• W4311481014 cites W1510940677 @default.
• W4311481014 cites W1521398910 @default.
• W4311481014 cites W1522658170 @default.
• W4311481014 cites W1592605778 @default.
• W4311481014 cites W1841876959 @default.
• W4311481014 cites W1938950791 @default.
• W4311481014 cites W1965357053 @default.
• W4311481014 cites W1974341459 @default.
• W4311481014 cites W1990658315 @default.
• W4311481014 cites W1991055989 @default.
• W4311481014 cites W2003516788 @default.
• W4311481014 cites W2005090163 @default.
• W4311481014 cites W2011886013 @default.
• W4311481014 cites W2012599065 @default.
• W4311481014 cites W2017391914 @default.
• W4311481014 cites W2019087520 @default.
• W4311481014 cites W2022974400 @default.
• W4311481014 cites W2035656569 @default.
• W4311481014 cites W2040417624 @default.
• W4311481014 cites W2041528665 @default.
• W4311481014 cites W2047633733 @default.
• W4311481014 cites W2055374672 @default.
• W4311481014 cites W2071123269 @default.
• W4311481014 cites W2087738338 @default.
• W4311481014 cites W2101852959 @default.
• W4311481014 cites W2117672149 @default.
• W4311481014 cites W2121647317 @default.
• W4311481014 cites W2148393598 @default.
• W4311481014 cites W2151420444 @default.
• W4311481014 cites W2156704489 @default.
• W4311481014 cites W2338595164 @default.
• W4311481014 cites W2379916129 @default.
• W4311481014 cites W2416223387 @default.
• W4311481014 cites W2522558171 @default.
• W4311481014 cites W2616048108 @default.
• W4311481014 cites W2742708327 @default.
• W4311481014 cites W2765437187 @default.
• W4311481014 cites W2791581727 @default.
• W4311481014 cites W2888014540 @default.
• W4311481014 cites W2899554378 @default.
• W4311481014 cites W2912405792 @default.
• W4311481014 cites W2916203459 @default.
• W4311481014 cites W2931523692 @default.
• W4311481014 cites W2983284863 @default.
• W4311481014 cites W2991735893 @default.
• W4311481014 cites W3096012088 @default.
• W4311481014 cites W3125661314 @default.
• W4311481014 cites W3190678892 @default.
• W4311481014 doi "https://doi.org/10.1128/spectrum.02017-22" @default.
• W4311481014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36515504" @default.
• W4311481014 hasPublicationYear "2023" @default.
• W4311481014 type Work @default.
• W4311481014 citedByCount "0" @default.
• W4311481014 crossrefType "journal-article" @default.
• W4311481014 hasAuthorship W4311481014A5001577023 @default.
• W4311481014 hasAuthorship W4311481014A5024197542 @default.
• W4311481014 hasAuthorship W4311481014A5028468364 @default.
• W4311481014 hasAuthorship W4311481014A5047908354 @default.
• W4311481014 hasAuthorship W4311481014A5055008485 @default.
• W4311481014 hasAuthorship W4311481014A5078750267 @default.
• W4311481014 hasAuthorship W4311481014A5088055959 @default.
• W4311481014 hasBestOaLocation W43114810141 @default.
• W4311481014 hasConcept C135870905 @default.
• W4311481014 hasConcept C181199279 @default.
• W4311481014 hasConcept C185592680 @default.
• W4311481014 hasConcept C192989942 @default.
• W4311481014 hasConcept C2777211131 @default.
• W4311481014 hasConcept C2777468819 @default.
• W4311481014 hasConcept C2777477808 @default.
• W4311481014 hasConcept C2779349466 @default.
• W4311481014 hasConcept C2779454058 @default.
• W4311481014 hasConcept C2779708577 @default.
• W4311481014 hasConcept C501593827 @default.
• W4311481014 hasConcept C515207424 @default.
• W4311481014 hasConcept C553450214 @default.
• W4311481014 hasConcept C55493867 @default.
• W4311481014 hasConcept C62231903 @default.
• W4311481014 hasConcept C86803240 @default.
• W4311481014 hasConceptScore W4311481014C135870905 @default.
• W4311481014 hasConceptScore W4311481014C181199279 @default.
• W4311481014 hasConceptScore W4311481014C185592680 @default.
• W4311481014 hasConceptScore W4311481014C192989942 @default.
• W4311481014 hasConceptScore W4311481014C2777211131 @default.
• W4311481014 hasConceptScore W4311481014C2777468819 @default.
• W4311481014 hasConceptScore W4311481014C2777477808 @default.
• W4311481014 hasConceptScore W4311481014C2779349466 @default.
• W4311481014 hasConceptScore W4311481014C2779454058 @default.
• W4311481014 hasConceptScore W4311481014C2779708577 @default.

• next