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- W4311498422 abstract "Here, we present optimized approaches to identify the efficiency of cancer cell phagocytosis by macrophages in vitro and in vivo. We describe the preparation and co-culture of macrophages and cancer cells, followed by in vitro phagocytosis assay using flow cytometry and confocal microscopy, respectively. We then detail the establishment of xenograft tumor mouse model and the in vivo detecting of phagocytosis efficiency by flow cytometry and qRT-PCR. This protocol provides a convenient way to assess macrophage-mediated phagocytosis of cancer cells. For complete details on the use and execution of this protocol, please refer to Xu et al.1." @default.
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- W4311498422 date "2023-03-01" @default.
- W4311498422 modified "2023-10-04" @default.
- W4311498422 title "Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo" @default.
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- W4311498422 doi "https://doi.org/10.1016/j.xpro.2022.101940" @default.
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