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- W4311556216 abstract "Single molecule localization microscopy relies on the precise quantification of the position of single dye emitters in a sample. This precision is improved by the number of photons that can be detected from each molecule. It is therefore recommendable to increase illumination times for the recording process. Particularly recording at cryogenic temperatures dramatically reduces photobleaching and thereby allows a massive increase in illumination times to several seconds. As a downside, microscope instabilities may well introduce jitter during such long illuminations, deteriorating the localization precision. In this paper, we theoretically demonstrate that a parallel recording of fiducial marker beads together with a novel fitting approach accounting for the full drift trajectory allows for largely eliminating drift effects for drift magnitudes of several hundred nanometers per frame." @default.
- W4311556216 created "2022-12-27" @default.
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- W4311556216 date "2022-12-13" @default.
- W4311556216 modified "2023-09-25" @default.
- W4311556216 title "Localization of fixed dipoles at high precision by accounting for sample drift during illumination" @default.
- W4311556216 doi "https://doi.org/10.48550/arxiv.2212.06545" @default.
- W4311556216 hasPublicationYear "2022" @default.
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