Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311651307> ?p ?o ?g. }
- W4311651307 endingPage "e0011011" @default.
- W4311651307 startingPage "e0011011" @default.
- W4311651307 abstract "Domestic dogs are primary reservoir hosts of Leishmania infantum, the agent of visceral leishmaniasis. Detecting dog infections is central to epidemiological inference, disease prevention, and veterinary practice. Error-free diagnostic procedures, however, are lacking, and the performance of those available is difficult to measure in the absence of fail-safe reference standards. Here, we illustrate how a hierarchical-modeling approach can be used to formally account for false-negative and false-positive results when investigating the process of Leishmania detection in dogs.We studied 294 field-sampled dogs of unknown infection status from a Leishmania-endemic region. We ran 350 parasitological tests (bone-marrow microscopy and culture) and 1,016 qPCR assays (blood, bone-marrow, and eye-swab samples with amplifiable DNA). Using replicate test results and site-occupancy models, we estimated (a) clinical sensitivity for each diagnostic procedure and (b) clinical specificity for qPCRs; parasitological tests were assumed 100% specific. Initial modeling revealed qPCR specificity < 94%; we tracked the source of this unexpected result to some qPCR plates having subtle signs of possible contamination. Using multi-model inference, we formally accounted for suspected plate contamination and estimated qPCR sensitivity at 49-53% across sample types and dog clinical conditions; qPCR specificity was high (95-96%), but fell to 81-82% for assays run in plates with suspected contamination. The sensitivity of parasitological procedures was low (~12-13%), but increased to ~33% (with substantial uncertainty) for bone-marrow culture in seriously-diseased dogs. Leishmania-infection frequency estimates (~49-50% across clinical conditions) were lower than observed (~60%).We provide statistical estimates of key performance parameters for five diagnostic procedures used to detect Leishmania in dogs. Low clinical sensitivies likely reflect the absence of Leishmania parasites/DNA in perhaps ~50-70% of samples drawn from infected dogs. Although qPCR performance was similar across sample types, non-invasive eye-swabs were overall less likely to contain amplifiable DNA. Finally, modeling was instrumental to discovering (and formally accounting for) possible qPCR-plate contamination; even with stringent negative/blank-control scoring, ~4-5% of positive qPCRs were most likely false-positives. This work shows, in sum, how hierarchical site-occupancy models can sharpen our understanding of the problem of diagnosing host infections with hard-to-detect pathogens including Leishmania." @default.
- W4311651307 created "2022-12-27" @default.
- W4311651307 creator A5003982251 @default.
- W4311651307 creator A5028762325 @default.
- W4311651307 creator A5040865925 @default.
- W4311651307 creator A5041775130 @default.
- W4311651307 creator A5047276471 @default.
- W4311651307 creator A5060209099 @default.
- W4311651307 creator A5060565057 @default.
- W4311651307 creator A5069012504 @default.
- W4311651307 creator A5071691409 @default.
- W4311651307 date "2022-12-16" @default.
- W4311651307 modified "2023-09-30" @default.
- W4311651307 title "Detecting Leishmania in dogs: A hierarchical-modeling approach to investigate the performance of parasitological and qPCR-based diagnostic procedures" @default.
- W4311651307 cites W1525998752 @default.
- W4311651307 cites W1876281013 @default.
- W4311651307 cites W1988148505 @default.
- W4311651307 cites W1988622420 @default.
- W4311651307 cites W2002070669 @default.
- W4311651307 cites W2003961169 @default.
- W4311651307 cites W2010504683 @default.
- W4311651307 cites W2014992919 @default.
- W4311651307 cites W2026004056 @default.
- W4311651307 cites W2034112342 @default.
- W4311651307 cites W2039696994 @default.
- W4311651307 cites W2042487212 @default.
- W4311651307 cites W2043542315 @default.
- W4311651307 cites W2046144222 @default.
- W4311651307 cites W2050345155 @default.
- W4311651307 cites W2051021300 @default.
- W4311651307 cites W2055888051 @default.
- W4311651307 cites W2064216140 @default.
- W4311651307 cites W2066081547 @default.
- W4311651307 cites W2079715126 @default.
- W4311651307 cites W2092793728 @default.
- W4311651307 cites W2094364398 @default.
- W4311651307 cites W2111494937 @default.
- W4311651307 cites W2113202773 @default.
- W4311651307 cites W2116006578 @default.
- W4311651307 cites W2117118633 @default.
- W4311651307 cites W2127056287 @default.
- W4311651307 cites W2128166966 @default.
- W4311651307 cites W2142072736 @default.
- W4311651307 cites W2159539317 @default.
- W4311651307 cites W2160713490 @default.
- W4311651307 cites W2168164420 @default.
- W4311651307 cites W2168287669 @default.
- W4311651307 cites W2225796577 @default.
- W4311651307 cites W2259789773 @default.
- W4311651307 cites W2546828523 @default.
- W4311651307 cites W2560754340 @default.
- W4311651307 cites W2582156763 @default.
- W4311651307 cites W2621867911 @default.
- W4311651307 cites W2724999405 @default.
- W4311651307 cites W2737772489 @default.
- W4311651307 cites W2761187146 @default.
- W4311651307 cites W2767323476 @default.
- W4311651307 cites W2768986192 @default.
- W4311651307 cites W2781983363 @default.
- W4311651307 cites W2783841473 @default.
- W4311651307 cites W2790893739 @default.
- W4311651307 cites W2886385847 @default.
- W4311651307 cites W2911518070 @default.
- W4311651307 cites W2911558340 @default.
- W4311651307 cites W2953928837 @default.
- W4311651307 cites W2979733184 @default.
- W4311651307 cites W2988946100 @default.
- W4311651307 cites W3006187346 @default.
- W4311651307 cites W3017634825 @default.
- W4311651307 cites W3083492088 @default.
- W4311651307 cites W3095802626 @default.
- W4311651307 cites W3177569723 @default.
- W4311651307 cites W3183295349 @default.
- W4311651307 cites W3196429043 @default.
- W4311651307 cites W4200058621 @default.
- W4311651307 cites W4200223154 @default.
- W4311651307 cites W4211073781 @default.
- W4311651307 cites W4211253687 @default.
- W4311651307 cites W4289782219 @default.
- W4311651307 doi "https://doi.org/10.1371/journal.pntd.0011011" @default.
- W4311651307 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36525465" @default.
- W4311651307 hasPublicationYear "2022" @default.
- W4311651307 type Work @default.
- W4311651307 citedByCount "0" @default.
- W4311651307 crossrefType "journal-article" @default.
- W4311651307 hasAuthorship W4311651307A5003982251 @default.
- W4311651307 hasAuthorship W4311651307A5028762325 @default.
- W4311651307 hasAuthorship W4311651307A5040865925 @default.
- W4311651307 hasAuthorship W4311651307A5041775130 @default.
- W4311651307 hasAuthorship W4311651307A5047276471 @default.
- W4311651307 hasAuthorship W4311651307A5060209099 @default.
- W4311651307 hasAuthorship W4311651307A5060565057 @default.
- W4311651307 hasAuthorship W4311651307A5069012504 @default.
- W4311651307 hasAuthorship W4311651307A5071691409 @default.
- W4311651307 hasBestOaLocation W43116513071 @default.
- W4311651307 hasConcept C105795698 @default.
- W4311651307 hasConcept C126322002 @default.
- W4311651307 hasConcept C136764020 @default.