FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311662904> ?p ?o ?g. }

• W4311662904 abstract "Abstract DNA methylation is a key epigenetic property that drives gene regulatory programs in development and disease. Current single-cell methods that produce high quality methylomes are expensive and low throughput without the aid of extensive automation. We previously described a proof-of-principle technique that enabled high cell throughput; however, it produced only low-coverage profiles and was a difficult protocol that required custom sequencing primers and recipes and frequently produced libraries with excessive adapter contamination. Here, we describe a greatly improved version that generates high-coverage profiles (~15-fold increase) using a robust protocol that does not require custom sequencing capabilities, includes multiple stopping points, and exhibits minimal adapter contamination. We demonstrate two versions of sciMETv2 on primary human cortex, a high coverage and rapid version, identifying distinct cell types using CH methylation patterns. These datasets are able to be directly integrated with one another as well as with existing snmC-seq2 datasets with little discernible bias. Finally, we demonstrate the ability to determine cell types using CG methylation alone, which is the dominant context for DNA methylation in most cell types other than neurons and the most applicable analysis outside of brain tissue." @default.
• W4311662904 created "2022-12-27" @default.
• W4311662904 creator A5004789657 @default.
• W4311662904 creator A5005184199 @default.
• W4311662904 creator A5010047624 @default.
• W4311662904 creator A5017281085 @default.
• W4311662904 creator A5022729320 @default.
• W4311662904 creator A5034834036 @default.
• W4311662904 creator A5042983202 @default.
• W4311662904 creator A5052656509 @default.
• W4311662904 creator A5086348500 @default.
• W4311662904 creator A5089616755 @default.
• W4311662904 date "2022-12-09" @default.
• W4311662904 modified "2023-10-03" @default.
• W4311662904 title "High-throughput robust single-cell DNA methylation profiling with sciMETv2" @default.
• W4311662904 cites W1631320694 @default.
• W4311662904 cites W2014677321 @default.
• W4311662904 cites W2036897871 @default.
• W4311662904 cites W2059099579 @default.
• W4311662904 cites W2065340354 @default.
• W4311662904 cites W2070021921 @default.
• W4311662904 cites W2070339716 @default.
• W4311662904 cites W2091811407 @default.
• W4311662904 cites W2131271579 @default.
• W4311662904 cites W2131374955 @default.
• W4311662904 cites W2139368808 @default.
• W4311662904 cites W2529781477 @default.
• W4311662904 cites W2580989000 @default.
• W4311662904 cites W2744040456 @default.
• W4311662904 cites W2797310920 @default.
• W4311662904 cites W2885355806 @default.
• W4311662904 cites W2902652978 @default.
• W4311662904 cites W2950909085 @default.
• W4311662904 cites W2951499602 @default.
• W4311662904 cites W3131801204 @default.
• W4311662904 cites W3137236105 @default.
• W4311662904 cites W3180741386 @default.
• W4311662904 cites W3203959602 @default.
• W4311662904 cites W4225512229 @default.
• W4311662904 doi "https://doi.org/10.1038/s41467-022-35374-3" @default.
• W4311662904 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36494343" @default.
• W4311662904 hasPublicationYear "2022" @default.
• W4311662904 type Work @default.
• W4311662904 citedByCount "7" @default.
• W4311662904 countsByYear W43116629042023 @default.
• W4311662904 crossrefType "journal-article" @default.
• W4311662904 hasAuthorship W4311662904A5004789657 @default.
• W4311662904 hasAuthorship W4311662904A5005184199 @default.
• W4311662904 hasAuthorship W4311662904A5010047624 @default.
• W4311662904 hasAuthorship W4311662904A5017281085 @default.
• W4311662904 hasAuthorship W4311662904A5022729320 @default.
• W4311662904 hasAuthorship W4311662904A5034834036 @default.
• W4311662904 hasAuthorship W4311662904A5042983202 @default.
• W4311662904 hasAuthorship W4311662904A5052656509 @default.
• W4311662904 hasAuthorship W4311662904A5086348500 @default.
• W4311662904 hasAuthorship W4311662904A5089616755 @default.
• W4311662904 hasBestOaLocation W43116629041 @default.
• W4311662904 hasConcept C104317684 @default.
• W4311662904 hasConcept C111919701 @default.
• W4311662904 hasConcept C150194340 @default.
• W4311662904 hasConcept C157764524 @default.
• W4311662904 hasConcept C177284502 @default.
• W4311662904 hasConcept C190727270 @default.
• W4311662904 hasConcept C33288867 @default.
• W4311662904 hasConcept C41008148 @default.
• W4311662904 hasConcept C41091548 @default.
• W4311662904 hasConcept C51679486 @default.
• W4311662904 hasConcept C54355233 @default.
• W4311662904 hasConcept C552990157 @default.
• W4311662904 hasConcept C555944384 @default.
• W4311662904 hasConcept C70721500 @default.
• W4311662904 hasConcept C86803240 @default.
• W4311662904 hasConcept C9390403 @default.
• W4311662904 hasConceptScore W4311662904C104317684 @default.
• W4311662904 hasConceptScore W4311662904C111919701 @default.
• W4311662904 hasConceptScore W4311662904C150194340 @default.
• W4311662904 hasConceptScore W4311662904C157764524 @default.
• W4311662904 hasConceptScore W4311662904C177284502 @default.
• W4311662904 hasConceptScore W4311662904C190727270 @default.
• W4311662904 hasConceptScore W4311662904C33288867 @default.
• W4311662904 hasConceptScore W4311662904C41008148 @default.
• W4311662904 hasConceptScore W4311662904C41091548 @default.
• W4311662904 hasConceptScore W4311662904C51679486 @default.
• W4311662904 hasConceptScore W4311662904C54355233 @default.
• W4311662904 hasConceptScore W4311662904C552990157 @default.
• W4311662904 hasConceptScore W4311662904C555944384 @default.
• W4311662904 hasConceptScore W4311662904C70721500 @default.
• W4311662904 hasConceptScore W4311662904C86803240 @default.
• W4311662904 hasConceptScore W4311662904C9390403 @default.
• W4311662904 hasFunder F4320337346 @default.
• W4311662904 hasFunder F4320337354 @default.
• W4311662904 hasIssue "1" @default.
• W4311662904 hasLocation W43116629041 @default.
• W4311662904 hasLocation W43116629042 @default.
• W4311662904 hasLocation W43116629043 @default.
• W4311662904 hasOpenAccess W4311662904 @default.
• W4311662904 hasPrimaryLocation W43116629041 @default.
• W4311662904 hasRelatedWork W1982470198 @default.
• W4311662904 hasRelatedWork W2024490794 @default.
• W4311662904 hasRelatedWork W2174782136 @default.

• next