FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311675032> ?p ?o ?g. }

• W4311675032 endingPage "17860" @default.
• W4311675032 startingPage "17853" @default.
• W4311675032 abstract "The urinary tract infections by antibiotic-resistant bacteria have been a serious public health problem and increase the healthcare costs. The conventional technologies of diagnosis and antimicrobial susceptibility testing (AST) relying on multiple culture-based assays are time-consuming and labor-intensive and thus compel the empirical antimicrobial therapies to be prescribed, fueling the prevalence of antimicrobial resistance. Herein, we propose an all-in-one Escherichia coli viability assay in an enclosed 3D microwell array chip, termed digital β-d-glucuronidase (GUS)-AST assay. It employs GUS, a specific metabolism-related enzyme, to convert the presence of E. coli into bright fluorescence. The random distribution of single bacteria in microwell array enables to quantify the E. coli concentrations by counting the positive microwells. We incorporate the most probable number with digital quantification to lower the limit of detection and expand the dynamic range to 7 orders. The digital GUS-AST assay is able to indicate the potency of antibiotics and determine the minimum inhibitory concentrations. A streamlined procedure of urine removal, bacterial separation, and digital GUS-AST is established to perform the direct analysis of bacteria population in urine. The sample-to-result workflow can be finished in 4.5 h with a limit of detection of 39 CFU/mL. With further development for additional pathogens and multiple antibiotic conditions, the digital GUS-AST assay could help physicians to prescribe timely targeted therapies for better patient outcomes and the minimum emergence of resistant bacteria." @default.
• W4311675032 created "2022-12-28" @default.
• W4311675032 creator A5022878865 @default.
• W4311675032 creator A5023363049 @default.
• W4311675032 creator A5023535715 @default.
• W4311675032 creator A5039544047 @default.
• W4311675032 creator A5049017147 @default.
• W4311675032 creator A5056190373 @default.
• W4311675032 creator A5090771026 @default.
• W4311675032 creator A5091151609 @default.
• W4311675032 date "2022-12-16" @default.
• W4311675032 modified "2023-09-28" @default.
• W4311675032 title "All-In-One <i>Escherichia coli</i> Viability Assay for Multi-dimensional Detection of Uncomplicated Urinary Tract Infections" @default.
• W4311675032 cites W1971643325 @default.
• W4311675032 cites W1993397663 @default.
• W4311675032 cites W2012432103 @default.
• W4311675032 cites W2023658600 @default.
• W4311675032 cites W2031240692 @default.
• W4311675032 cites W2085801321 @default.
• W4311675032 cites W2096369133 @default.
• W4311675032 cites W2101182625 @default.
• W4311675032 cites W2102806675 @default.
• W4311675032 cites W2110901986 @default.
• W4311675032 cites W2111491330 @default.
• W4311675032 cites W2118115068 @default.
• W4311675032 cites W2155448001 @default.
• W4311675032 cites W2157579931 @default.
• W4311675032 cites W2157869310 @default.
• W4311675032 cites W2165186144 @default.
• W4311675032 cites W2471494121 @default.
• W4311675032 cites W2509790828 @default.
• W4311675032 cites W2582182347 @default.
• W4311675032 cites W2586207359 @default.
• W4311675032 cites W2744332922 @default.
• W4311675032 cites W2761083139 @default.
• W4311675032 cites W2763809335 @default.
• W4311675032 cites W2804051292 @default.
• W4311675032 cites W2814001856 @default.
• W4311675032 cites W2885002399 @default.
• W4311675032 cites W2901606383 @default.
• W4311675032 cites W2930500531 @default.
• W4311675032 cites W2943512397 @default.
• W4311675032 cites W2944188517 @default.
• W4311675032 cites W2974106063 @default.
• W4311675032 cites W3076128127 @default.
• W4311675032 doi "https://doi.org/10.1021/acs.analchem.2c03604" @default.
• W4311675032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36524619" @default.
• W4311675032 hasPublicationYear "2022" @default.
• W4311675032 type Work @default.
• W4311675032 citedByCount "2" @default.
• W4311675032 countsByYear W43116750322023 @default.
• W4311675032 crossrefType "journal-article" @default.
• W4311675032 hasAuthorship W4311675032A5022878865 @default.
• W4311675032 hasAuthorship W4311675032A5023363049 @default.
• W4311675032 hasAuthorship W4311675032A5023535715 @default.
• W4311675032 hasAuthorship W4311675032A5039544047 @default.
• W4311675032 hasAuthorship W4311675032A5049017147 @default.
• W4311675032 hasAuthorship W4311675032A5056190373 @default.
• W4311675032 hasAuthorship W4311675032A5090771026 @default.
• W4311675032 hasAuthorship W4311675032A5091151609 @default.
• W4311675032 hasConcept C104317684 @default.
• W4311675032 hasConcept C119128265 @default.
• W4311675032 hasConcept C176947019 @default.
• W4311675032 hasConcept C185592680 @default.
• W4311675032 hasConcept C2780026642 @default.
• W4311675032 hasConcept C2908647359 @default.
• W4311675032 hasConcept C43617362 @default.
• W4311675032 hasConcept C4937899 @default.
• W4311675032 hasConcept C501593827 @default.
• W4311675032 hasConcept C523546767 @default.
• W4311675032 hasConcept C54355233 @default.
• W4311675032 hasConcept C547475151 @default.
• W4311675032 hasConcept C55493867 @default.
• W4311675032 hasConcept C71924100 @default.
• W4311675032 hasConcept C86803240 @default.
• W4311675032 hasConcept C89423630 @default.
• W4311675032 hasConcept C94665300 @default.
• W4311675032 hasConcept C99454951 @default.
• W4311675032 hasConceptScore W4311675032C104317684 @default.
• W4311675032 hasConceptScore W4311675032C119128265 @default.
• W4311675032 hasConceptScore W4311675032C176947019 @default.
• W4311675032 hasConceptScore W4311675032C185592680 @default.
• W4311675032 hasConceptScore W4311675032C2780026642 @default.
• W4311675032 hasConceptScore W4311675032C2908647359 @default.
• W4311675032 hasConceptScore W4311675032C43617362 @default.
• W4311675032 hasConceptScore W4311675032C4937899 @default.
• W4311675032 hasConceptScore W4311675032C501593827 @default.
• W4311675032 hasConceptScore W4311675032C523546767 @default.
• W4311675032 hasConceptScore W4311675032C54355233 @default.
• W4311675032 hasConceptScore W4311675032C547475151 @default.
• W4311675032 hasConceptScore W4311675032C55493867 @default.
• W4311675032 hasConceptScore W4311675032C71924100 @default.
• W4311675032 hasConceptScore W4311675032C86803240 @default.
• W4311675032 hasConceptScore W4311675032C89423630 @default.
• W4311675032 hasConceptScore W4311675032C94665300 @default.
• W4311675032 hasConceptScore W4311675032C99454951 @default.
• W4311675032 hasFunder F4320321001 @default.
• W4311675032 hasIssue "51" @default.

• next