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• W4311688064 endingPage "2232" @default.
• W4311688064 startingPage "2232" @default.
• W4311688064 abstract "Low-cost diagnostic tools for point-of-care immunoassays, such as the paper-based enzyme-linked immunoassay (ELISA), have become increasingly important, especially so in the recent COVID-19 pandemic. ELISA is the gold-standard antibody/antigen sensing method. This paper reports an easy-to-fabricate nitrocellulose (NC) paper plate, coupled with a desktop scanner for ELISA, which provides a higher protein immobilization efficiency than the conventional cellulose paper-based ELISA platforms. The experiments were performed using spiked samples for the direct ELISA of rabbit IgG with a limit of detection (LOD) of 1.016 μg/mL, in a measurement range of 10 ng/mL to 1 mg/mL, and for the sandwich ELISA of sperm protein (SP-10) with an LOD of 88.8 ng/mL, in a measurement range of 1 ng/mL to 100 μg/mL. The described fabrication method, based on laser-cutting, is a highly flexible one-step laser micromachining process, which enables the rapid production of low-cost NC paper-based multi-well plates with different sizes for the ELISA measurements." @default.
• W4311688064 created "2022-12-28" @default.
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• W4311688064 date "2022-12-16" @default.
• W4311688064 modified "2023-10-15" @default.
• W4311688064 title "A Nitrocellulose Paper-Based Multi-Well Plate for Point-of-Care ELISA" @default.
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• W4311688064 doi "https://doi.org/10.3390/mi13122232" @default.
• W4311688064 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36557531" @default.
• W4311688064 hasPublicationYear "2022" @default.
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