Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311688506> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W4311688506 endingPage "1649" @default.
- W4311688506 startingPage "1649" @default.
- W4311688506 abstract "Glutathione is of great significance in pharmaceutical and health fields, and one-step synthesis of reduced glutathione by glutathione bifunctional synthase has become a focus of research. The stability of glutathione bifunctional synthase is generally poor and urgently needs to be modified. The B-factor strategy and un/folding free energy calculation were both applied to enhance the thermal stability of glutathione bifunctional synthase from Streptococcus agalactiae (GshFSA). Based on the concept of B-factor strategy, we calculated the B-factor by molecular dynamics simulation to find flexible residues, performed point saturation mutations and high-throughput screening. At the same time, we also calculated the un/folding free energy of GshFSA and performed the point mutations. The optimal mutant from the B-factor strategy was R270S, which had a 2.62-fold increase in half-life period compared to the wild type, and the Q406M was the optimal mutant from the un/folding free energy calculation, with a 3.02-fold increase in half-life period. Both of them have provided a mechanistic explanation." @default.
- W4311688506 created "2022-12-28" @default.
- W4311688506 creator A5029543328 @default.
- W4311688506 creator A5036965780 @default.
- W4311688506 creator A5048522413 @default.
- W4311688506 creator A5049352771 @default.
- W4311688506 creator A5057168565 @default.
- W4311688506 creator A5067180705 @default.
- W4311688506 creator A5079318963 @default.
- W4311688506 creator A5081229366 @default.
- W4311688506 date "2022-12-15" @default.
- W4311688506 modified "2023-10-05" @default.
- W4311688506 title "Enhancing the Thermal Stability of Glutathione Bifunctional Synthase by B-Factor Strategy and Un/Folding Free Energy Calculation" @default.
- W4311688506 cites W1869803502 @default.
- W4311688506 cites W1979928186 @default.
- W4311688506 cites W1987196385 @default.
- W4311688506 cites W2012717668 @default.
- W4311688506 cites W2014159272 @default.
- W4311688506 cites W2081245251 @default.
- W4311688506 cites W2082432950 @default.
- W4311688506 cites W2087564275 @default.
- W4311688506 cites W2105440994 @default.
- W4311688506 cites W2123906667 @default.
- W4311688506 cites W2140197221 @default.
- W4311688506 cites W2300649977 @default.
- W4311688506 cites W2558718647 @default.
- W4311688506 cites W2607570365 @default.
- W4311688506 cites W2806393360 @default.
- W4311688506 cites W2920817727 @default.
- W4311688506 cites W3039646408 @default.
- W4311688506 cites W3110621523 @default.
- W4311688506 cites W3177828909 @default.
- W4311688506 cites W3216851640 @default.
- W4311688506 cites W4292865836 @default.
- W4311688506 cites W4293555427 @default.
- W4311688506 cites W4297249271 @default.
- W4311688506 cites W4304480239 @default.
- W4311688506 doi "https://doi.org/10.3390/catal12121649" @default.
- W4311688506 hasPublicationYear "2022" @default.
- W4311688506 type Work @default.
- W4311688506 citedByCount "1" @default.
- W4311688506 countsByYear W43116885062023 @default.
- W4311688506 crossrefType "journal-article" @default.
- W4311688506 hasAuthorship W4311688506A5029543328 @default.
- W4311688506 hasAuthorship W4311688506A5036965780 @default.
- W4311688506 hasAuthorship W4311688506A5048522413 @default.
- W4311688506 hasAuthorship W4311688506A5049352771 @default.
- W4311688506 hasAuthorship W4311688506A5057168565 @default.
- W4311688506 hasAuthorship W4311688506A5067180705 @default.
- W4311688506 hasAuthorship W4311688506A5079318963 @default.
- W4311688506 hasAuthorship W4311688506A5081229366 @default.
- W4311688506 hasBestOaLocation W43116885061 @default.
- W4311688506 hasConcept C112243037 @default.
- W4311688506 hasConcept C119599485 @default.
- W4311688506 hasConcept C127413603 @default.
- W4311688506 hasConcept C161790260 @default.
- W4311688506 hasConcept C181199279 @default.
- W4311688506 hasConcept C185592680 @default.
- W4311688506 hasConcept C2776545253 @default.
- W4311688506 hasConcept C2780443040 @default.
- W4311688506 hasConcept C538909803 @default.
- W4311688506 hasConcept C55493867 @default.
- W4311688506 hasConceptScore W4311688506C112243037 @default.
- W4311688506 hasConceptScore W4311688506C119599485 @default.
- W4311688506 hasConceptScore W4311688506C127413603 @default.
- W4311688506 hasConceptScore W4311688506C161790260 @default.
- W4311688506 hasConceptScore W4311688506C181199279 @default.
- W4311688506 hasConceptScore W4311688506C185592680 @default.
- W4311688506 hasConceptScore W4311688506C2776545253 @default.
- W4311688506 hasConceptScore W4311688506C2780443040 @default.
- W4311688506 hasConceptScore W4311688506C538909803 @default.
- W4311688506 hasConceptScore W4311688506C55493867 @default.
- W4311688506 hasFunder F4320335777 @default.
- W4311688506 hasIssue "12" @default.
- W4311688506 hasLocation W43116885061 @default.
- W4311688506 hasOpenAccess W4311688506 @default.
- W4311688506 hasPrimaryLocation W43116885061 @default.
- W4311688506 hasRelatedWork W2065583757 @default.
- W4311688506 hasRelatedWork W2312657253 @default.
- W4311688506 hasRelatedWork W2343617961 @default.
- W4311688506 hasRelatedWork W2395122550 @default.
- W4311688506 hasRelatedWork W2407423735 @default.
- W4311688506 hasRelatedWork W2950470046 @default.
- W4311688506 hasRelatedWork W2951492779 @default.
- W4311688506 hasRelatedWork W2952163184 @default.
- W4311688506 hasRelatedWork W2952886094 @default.
- W4311688506 hasRelatedWork W2953087550 @default.
- W4311688506 hasVolume "12" @default.
- W4311688506 isParatext "false" @default.
- W4311688506 isRetracted "false" @default.
- W4311688506 workType "article" @default.