Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311703029> ?p ?o ?g. }
- W4311703029 abstract "ABSTRACT Gut stem cells are accessible by biopsy and propagate robustly in culture, offering an invaluable resource for autologous cell therapies. Insulin-producing cells can be induced in mouse gut, but it has not been possible to generate abundant and durable insulin-secreting cells from human gut tissues to evaluate their potential as a cell therapy for diabetes. Here we describe a protocol to differentiate cultured human gastric stem cells (hGSCs) into pancreatic islet-like organoids containing gastric insulin-secreting (GINS) cells that resemble β-cells in molecular hallmarks and function. Sequential activation of the inducing factors NGN3 and PDX1-MAFA led hGSCs onto a novel differentiation path, including a SOX4 High endocrine and Galanin High GINS precursor, before adopting β-cell identity, at efficiencies close to 70%. GINS organoids acquired glucose-stimulated insulin secretion in 10 days and restored glucose homeostasis for over 100 days in diabetic mice after transplantation, providing proof of concept for a new approach to treat diabetes." @default.
- W4311703029 created "2022-12-28" @default.
- W4311703029 creator A5005214633 @default.
- W4311703029 creator A5005242574 @default.
- W4311703029 creator A5006713946 @default.
- W4311703029 creator A5007566255 @default.
- W4311703029 creator A5012615383 @default.
- W4311703029 creator A5014870189 @default.
- W4311703029 creator A5018960874 @default.
- W4311703029 creator A5019991320 @default.
- W4311703029 creator A5031444445 @default.
- W4311703029 creator A5042522014 @default.
- W4311703029 creator A5051145224 @default.
- W4311703029 creator A5068741272 @default.
- W4311703029 date "2022-12-16" @default.
- W4311703029 modified "2023-10-14" @default.
- W4311703029 title "Restoring glucose homeostasis with stomach-derived human insulin-secreting organoids" @default.
- W4311703029 cites W1910612799 @default.
- W4311703029 cites W1965157206 @default.
- W4311703029 cites W1982603620 @default.
- W4311703029 cites W1984883254 @default.
- W4311703029 cites W2002513358 @default.
- W4311703029 cites W2009193072 @default.
- W4311703029 cites W2035618305 @default.
- W4311703029 cites W2036682769 @default.
- W4311703029 cites W2041777158 @default.
- W4311703029 cites W2044801034 @default.
- W4311703029 cites W2067186404 @default.
- W4311703029 cites W2081276394 @default.
- W4311703029 cites W2093065606 @default.
- W4311703029 cites W2097387056 @default.
- W4311703029 cites W2110509725 @default.
- W4311703029 cites W2122986690 @default.
- W4311703029 cites W2126780067 @default.
- W4311703029 cites W2130595746 @default.
- W4311703029 cites W2132484969 @default.
- W4311703029 cites W2159155233 @default.
- W4311703029 cites W2284106784 @default.
- W4311703029 cites W2526262591 @default.
- W4311703029 cites W2582033455 @default.
- W4311703029 cites W2608182982 @default.
- W4311703029 cites W2611779918 @default.
- W4311703029 cites W2616922646 @default.
- W4311703029 cites W2747877289 @default.
- W4311703029 cites W2766437506 @default.
- W4311703029 cites W2794480084 @default.
- W4311703029 cites W2805425765 @default.
- W4311703029 cites W2811387400 @default.
- W4311703029 cites W2890033199 @default.
- W4311703029 cites W2901208726 @default.
- W4311703029 cites W2901254075 @default.
- W4311703029 cites W2911604931 @default.
- W4311703029 cites W2914432255 @default.
- W4311703029 cites W2982741813 @default.
- W4311703029 cites W2994810741 @default.
- W4311703029 cites W2994879170 @default.
- W4311703029 cites W3068518905 @default.
- W4311703029 cites W3087653149 @default.
- W4311703029 cites W3125204220 @default.
- W4311703029 cites W3133476978 @default.
- W4311703029 cites W3133960398 @default.
- W4311703029 cites W3175752499 @default.
- W4311703029 cites W3185481711 @default.
- W4311703029 cites W3204946529 @default.
- W4311703029 cites W3214753991 @default.
- W4311703029 cites W4211070901 @default.
- W4311703029 cites W4226209268 @default.
- W4311703029 doi "https://doi.org/10.1101/2022.12.15.520488" @default.
- W4311703029 hasPublicationYear "2022" @default.
- W4311703029 type Work @default.
- W4311703029 citedByCount "0" @default.
- W4311703029 crossrefType "posted-content" @default.
- W4311703029 hasAuthorship W4311703029A5005214633 @default.
- W4311703029 hasAuthorship W4311703029A5005242574 @default.
- W4311703029 hasAuthorship W4311703029A5006713946 @default.
- W4311703029 hasAuthorship W4311703029A5007566255 @default.
- W4311703029 hasAuthorship W4311703029A5012615383 @default.
- W4311703029 hasAuthorship W4311703029A5014870189 @default.
- W4311703029 hasAuthorship W4311703029A5018960874 @default.
- W4311703029 hasAuthorship W4311703029A5019991320 @default.
- W4311703029 hasAuthorship W4311703029A5031444445 @default.
- W4311703029 hasAuthorship W4311703029A5042522014 @default.
- W4311703029 hasAuthorship W4311703029A5051145224 @default.
- W4311703029 hasAuthorship W4311703029A5068741272 @default.
- W4311703029 hasBestOaLocation W43117030291 @default.
- W4311703029 hasConcept C103395026 @default.
- W4311703029 hasConcept C10882517 @default.
- W4311703029 hasConcept C126322002 @default.
- W4311703029 hasConcept C134018914 @default.
- W4311703029 hasConcept C165220095 @default.
- W4311703029 hasConcept C2777180221 @default.
- W4311703029 hasConcept C2777391703 @default.
- W4311703029 hasConcept C2778764654 @default.
- W4311703029 hasConcept C2778881409 @default.
- W4311703029 hasConcept C2779050171 @default.
- W4311703029 hasConcept C2779306644 @default.
- W4311703029 hasConcept C28328180 @default.
- W4311703029 hasConcept C2911091166 @default.
- W4311703029 hasConcept C3018667095 @default.
- W4311703029 hasConcept C31695470 @default.