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- W4311738480 abstract "Abstract This study evaluated whether cell sheets containing a network of lymphatic endothelial cells (LECs) promoted lymphangiogenesis after transplantation in vivo. Cell sheets with a LEC network were constructed by co-culturing LECs and adipose-derived stem cells (ASCs) on temperature-responsive culture dishes. A cell ratio of 3:2 (vs. 1:4) generated networks with more branches and longer branch lengths. LEC-derived lymphatic vessels were observed 2 weeks after transplantation of a three-layered cell sheet construct onto rat gluteal muscle. Lymphatic vessel number, diameter and depth were greatest for a construct comprising two ASC sheets stacked on a LEC/ASC (3:2 ratio) sheet. Transplantation of this construct in a rat model of femoral lymphangiectomy led to the formation of functional lymphatic vessels containing both transplanted and host LECs. Further development of this technique may lead to a new method of promoting lymphangiogenesis." @default.
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- W4311738480 date "2022-12-15" @default.
- W4311738480 modified "2023-10-14" @default.
- W4311738480 title "Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels" @default.
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- W4311738480 doi "https://doi.org/10.1038/s41598-022-26041-0" @default.
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