Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311752054> ?p ?o ?g. }
- W4311752054 endingPage "35" @default.
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- W4311752054 abstract "In this study, the galectin (OrGal) was purified from the grub of rhinoceros beetle (Oryctes rhinoceros) (Coleoptera: Scarabaeidae) using lactose-affinity chromatography and its molecular mass was determined as 191 kDa. The OrGal binds lactose, galactose and BSM. It is a heterotrimer whose IEF were ∼5–5.5 (50 kDa), 7 (75 kDa) and 8 (95 kDa). The OrGal was glycine-rich and showed peptide-homology to galectin and mitogenic lectin. From the isolated human PBMC, CD4+ T and CD19+ B cells were sorted and confirmed with CD markers. The OrGal was mitogenic to T-cells but not B cells (24 h and 48 h), and this activity was repressed by lactose. Expression of inflammatory mediators showed that OrGal upregulated IL-2 and IL-4, and downregulated IFN-γ, IL-6 and GM-CSF in LPS-primed-T-cells. In the LPS-primed-monocytes, OrGal downregulated NF-κB, TNF-α, IL-1β, IL-6, IL-12 and NO. The de novo transcriptomics identified transcripts (PB 3585.1 & PB 3817.1) showing identity to Coleopteran galectin-4, − 6, − 8 and − 9, in which galectin-8 & − 4 showed high similarities. Mass spectrometry sequences of OrGal and its subunit exhibited a considerable identity to PB 3585.1 (galectin-8) & PB 3817.1 (galectin-4). The in silico models of above two transcripts interacted with lactose and galactose." @default.
- W4311752054 created "2022-12-28" @default.
- W4311752054 creator A5014475799 @default.
- W4311752054 creator A5015234243 @default.
- W4311752054 creator A5023817711 @default.
- W4311752054 creator A5026781194 @default.
- W4311752054 creator A5032647827 @default.
- W4311752054 creator A5048938917 @default.
- W4311752054 creator A5085918558 @default.
- W4311752054 date "2023-02-01" @default.
- W4311752054 modified "2023-09-25" @default.
- W4311752054 title "Insect galectin stimulates the human CD4+ T cell proliferation by regulating inflammation (T cell and monocyte) through Th2 immune response" @default.
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