Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311779849> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W4311779849 endingPage "340679" @default.
- W4311779849 startingPage "340679" @default.
- W4311779849 abstract "s: To overcome the limitation of polymerase chain reaction (PCR), isothermal amplification methods such as thermophilic helicase-dependent amplification (tHDA) have been developed. However, formation of primer dimer due to the single amplification temperature are major problems of tHDA. When cross-dimerization of forward and reverse primer occurred, false-positive results can be found on the lateral flow assay (LFA) which is one of the major detection methods widely used as a point of care diagnosis. Therefore, specific method of detecting only the target amplicon is required.In this study, a tHDA-based CRISPR/Cas12a system was developed to detect low levels of Escherichia coli O157:H7 in fresh salad mix without the false-positive results produced by primer dimers. For the comparison of the effect in eliminating false-positive results by CRISPR/Cas12a system, LFA was also evaluated. The tHDA-based CRISPR/Cas12a system detected as low as 101 CFU/mL E. coli O157:H7 in bacterial pure culture. In LFA false-positive results were produced due to the primer dimer, whereas the primer dimer produced by tHDA was not detected in the CRISPR/Cas12a system. These results indicated that the CRISPR/Cas12a system eliminated the formation of primer dimer. In fresh salad mix, the tHDA-based CRISPR/Cas12a system combined with the filter concentration method detected 103 CFU/g E. coli O157:H7.This study was the first to amplify stx2 of E. coli O157:H7 with tHDA as an isothermal amplification method and detected the amplicon without false-positive results by combining tHDA with CRISPR/Cas12a. Therefore, this study showed great potential for detecting low levels of E. coli O157:H7 present in fresh salad mix." @default.
- W4311779849 created "2022-12-28" @default.
- W4311779849 creator A5034003059 @default.
- W4311779849 creator A5046966981 @default.
- W4311779849 creator A5059476948 @default.
- W4311779849 date "2023-01-01" @default.
- W4311779849 modified "2023-10-01" @default.
- W4311779849 title "Thermophilic helicase-dependent amplification-based CRISPR/Cas12a system: Detection of stx2 in Escherichia coli O157:H7 by controlling primer dimers" @default.
- W4311779849 cites W1972635736 @default.
- W4311779849 cites W1982228459 @default.
- W4311779849 cites W2008931521 @default.
- W4311779849 cites W2044051094 @default.
- W4311779849 cites W2050047163 @default.
- W4311779849 cites W2060565971 @default.
- W4311779849 cites W2073331390 @default.
- W4311779849 cites W2100538295 @default.
- W4311779849 cites W2104663976 @default.
- W4311779849 cites W2150186914 @default.
- W4311779849 cites W2592615709 @default.
- W4311779849 cites W2615187495 @default.
- W4311779849 cites W2755987600 @default.
- W4311779849 cites W2765818676 @default.
- W4311779849 cites W2893202922 @default.
- W4311779849 cites W2909571268 @default.
- W4311779849 cites W2979345235 @default.
- W4311779849 cites W3005733211 @default.
- W4311779849 cites W3006168659 @default.
- W4311779849 cites W3033071977 @default.
- W4311779849 cites W3037247917 @default.
- W4311779849 cites W3126789423 @default.
- W4311779849 cites W3131141423 @default.
- W4311779849 cites W3164698711 @default.
- W4311779849 cites W3191520269 @default.
- W4311779849 cites W3194617111 @default.
- W4311779849 cites W3194761412 @default.
- W4311779849 doi "https://doi.org/10.1016/j.aca.2022.340679" @default.
- W4311779849 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36628706" @default.
- W4311779849 hasPublicationYear "2023" @default.
- W4311779849 type Work @default.
- W4311779849 citedByCount "5" @default.
- W4311779849 countsByYear W43117798492023 @default.
- W4311779849 crossrefType "journal-article" @default.
- W4311779849 hasAuthorship W4311779849A5034003059 @default.
- W4311779849 hasAuthorship W4311779849A5046966981 @default.
- W4311779849 hasAuthorship W4311779849A5059476948 @default.
- W4311779849 hasConcept C104317684 @default.
- W4311779849 hasConcept C139713532 @default.
- W4311779849 hasConcept C153911025 @default.
- W4311779849 hasConcept C178790620 @default.
- W4311779849 hasConcept C185592680 @default.
- W4311779849 hasConcept C2777563447 @default.
- W4311779849 hasConcept C49105822 @default.
- W4311779849 hasConcept C547475151 @default.
- W4311779849 hasConcept C552990157 @default.
- W4311779849 hasConcept C55493867 @default.
- W4311779849 hasConcept C60635243 @default.
- W4311779849 hasConcept C8185291 @default.
- W4311779849 hasConcept C86803240 @default.
- W4311779849 hasConcept C98108389 @default.
- W4311779849 hasConceptScore W4311779849C104317684 @default.
- W4311779849 hasConceptScore W4311779849C139713532 @default.
- W4311779849 hasConceptScore W4311779849C153911025 @default.
- W4311779849 hasConceptScore W4311779849C178790620 @default.
- W4311779849 hasConceptScore W4311779849C185592680 @default.
- W4311779849 hasConceptScore W4311779849C2777563447 @default.
- W4311779849 hasConceptScore W4311779849C49105822 @default.
- W4311779849 hasConceptScore W4311779849C547475151 @default.
- W4311779849 hasConceptScore W4311779849C552990157 @default.
- W4311779849 hasConceptScore W4311779849C55493867 @default.
- W4311779849 hasConceptScore W4311779849C60635243 @default.
- W4311779849 hasConceptScore W4311779849C8185291 @default.
- W4311779849 hasConceptScore W4311779849C86803240 @default.
- W4311779849 hasConceptScore W4311779849C98108389 @default.
- W4311779849 hasFunder F4320322033 @default.
- W4311779849 hasFunder F4320322068 @default.
- W4311779849 hasLocation W43117798491 @default.
- W4311779849 hasLocation W43117798492 @default.
- W4311779849 hasOpenAccess W4311779849 @default.
- W4311779849 hasPrimaryLocation W43117798491 @default.
- W4311779849 hasRelatedWork W2108674897 @default.
- W4311779849 hasRelatedWork W2790972692 @default.
- W4311779849 hasRelatedWork W3109667847 @default.
- W4311779849 hasRelatedWork W3123310325 @default.
- W4311779849 hasRelatedWork W3205628816 @default.
- W4311779849 hasRelatedWork W3206408081 @default.
- W4311779849 hasRelatedWork W4206424404 @default.
- W4311779849 hasRelatedWork W4211003101 @default.
- W4311779849 hasRelatedWork W4225319205 @default.
- W4311779849 hasRelatedWork W4311779849 @default.
- W4311779849 hasVolume "1239" @default.
- W4311779849 isParatext "false" @default.
- W4311779849 isRetracted "false" @default.
- W4311779849 workType "article" @default.