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- W4311784838 endingPage "2048" @default.
- W4311784838 startingPage "2048" @default.
- W4311784838 abstract "Metagenomic Next Generation Sequencing (mNGS) allows the evaluation of complex microbial communities, avoiding isolation and cultivation of each microbial species, and does not require prior knowledge of the microbial sequences present in the sample. Applications of mNGS include virome characterization, new virus discovery and full-length viral genome reconstruction, either from virus preparations enriched in culture or directly from clinical and environmental specimens. Here, we systematically reviewed studies that describe novel virus identification through mNGS from samples of different origin (plant, animal and environment). Without imposing time limits to the search, 379 publications were identified that met the search parameters. Sample types, geographical origin, enrichment and nucleic acid extraction methods, sequencing platforms, bioinformatic analytical steps and identified viral families were described. The review highlights mNGS as a feasible method for novel virus discovery from samples of different origins, describes which kind of heterogeneous experimental and analytical protocols are currently used and provides useful information such as the different commercial kits used for the purification of nucleic acids and bioinformatics analytical pipelines." @default.
- W4311784838 created "2022-12-28" @default.
- W4311784838 creator A5001240587 @default.
- W4311784838 creator A5002313919 @default.
- W4311784838 creator A5008504942 @default.
- W4311784838 creator A5010513377 @default.
- W4311784838 creator A5070231483 @default.
- W4311784838 date "2022-12-07" @default.
- W4311784838 modified "2023-10-17" @default.
- W4311784838 title "Novel Virus Identification through Metagenomics: A Systematic Review" @default.
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