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• W4311792359 endingPage "3178" @default.
• W4311792359 startingPage "3178" @default.
• W4311792359 abstract "Bacterial halo blight (BHB) is a bacterial disease, caused by Pseudomonas syringae pv. garcae, which has been gaining prominence in the main coffee-producing regions. Chemical control of this disease increases production costs and is environmentally undesirable. In this scenario, the development of new cultivars resistant to BHB is the most economical and sustainable alternative. Marker-Assisted Selection (MAS) is an appropriate strategy to assist breeding programs for resistant genotype selection. In a previous Genome-Wide Association Study (GWAS) for C. arabica and P. syringae pv. garcae interaction, we identified a locus, probably linked to qualitative resistance to the pathogen. In this work, we developed and validated a pair of Allele-Specific-Polymerase Chain Reaction (AS-PCR) primers for this locus in C. arabica breeding populations. This pair of AS-PCR primers, called Psg_QL1, was tested both in a backcross (BC) (n = 38) and in an F2 population (n = 138) segregating for resistance to BHB. The linkage between the Psg_QL1 marker and qualitative resistance showed an accuracy of 93.75%. Our results demonstrated that the Psg_QL1 marker can be applied in MAS in a robust, simple, fast, and low-cost way." @default.
• W4311792359 created "2022-12-28" @default.
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• W4311792359 date "2022-12-15" @default.
• W4311792359 modified "2023-10-14" @default.
• W4311792359 title "Development and Validation of an Allele-Specific Marker for Resistance to Bacterial Halo Blight in Coffea arabica" @default.
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• W4311792359 doi "https://doi.org/10.3390/agronomy12123178" @default.
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