FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311873368> ?p ?o ?g. }

• W4311873368 abstract "Abstract Background Peptidoglycan recognition protein-3 (PGRP3) is a pattern recognition receptor that binds peptidoglycan to elicit an immune response. How PGRP3 mediates these immunomodulatory effects remains unknown. Identifying the proteins that interact with PGRP3 will help begin to address this important knowledge gap. Therefore, in this study our objective was to identify and validate novel PGRP3-protein interactions. Methods PGRP3-protein interactions were identified using a yeast two-hybrid system. PGRP3 cloned into a pGBKT7 DNA-BD vector and transformed into a Y2HGold yeast strain served as the bait. A normalized universal human cDNA library cloned in a pGADT7 AD vector and pre-transformed into an Y187 yeast strain served as the prey. Y2HGold and Y187 yeast strains were mated and diploids were plated on selective media. True positive clones were sent in for DNA sequencing and corresponding proteins were identified through NCBI Blast. Two proteins were selected for validation. His-tagged PGRP3 expressing 293T cells were then co-transfected with expression vectors containing the identified proteins tagged with c-Myc. Lysates from co-transfected cells were subjected to anti-His and anti-Myc co-immunoprecipitation and analyzed with western blots. Results Four unique proteins— MBNL3, RBP5, ATXN2, and GPATCH8—were identified from our yeast two-hybrid screen. Co-transfection and co-immunoprecipitation assays successfully confirmed MBNL3 and RBP5 as positive interactors with PGRP3. Conclusions Based on the known functions of MBNL3, RBP5, ATXN2, and GPATCH8, we hypothesize that PGRP3 may be involved in RNA processing, endocytic trafficking, and PPARγ pathways related to innate immunity." @default.
• W4311873368 created "2023-01-02" @default.
• W4311873368 creator A5000551183 @default.
• W4311873368 creator A5008515166 @default.
• W4311873368 creator A5018736862 @default.
• W4311873368 creator A5025229383 @default.
• W4311873368 creator A5069150690 @default.
• W4311873368 creator A5075897372 @default.
• W4311873368 date "2022-12-18" @default.
• W4311873368 modified "2023-10-12" @default.
• W4311873368 title "Identification of novel PGRP3 protein -protein interactions using yeast two hybrid system" @default.
• W4311873368 cites W1490939051 @default.
• W4311873368 cites W1496748157 @default.
• W4311873368 cites W1611787727 @default.
• W4311873368 cites W1860612338 @default.
• W4311873368 cites W1970769542 @default.
• W4311873368 cites W1973615836 @default.
• W4311873368 cites W1991163497 @default.
• W4311873368 cites W1993442297 @default.
• W4311873368 cites W1997887889 @default.
• W4311873368 cites W2029845836 @default.
• W4311873368 cites W2033368676 @default.
• W4311873368 cites W2037134815 @default.
• W4311873368 cites W2052040911 @default.
• W4311873368 cites W2064771691 @default.
• W4311873368 cites W2066163347 @default.
• W4311873368 cites W2067141301 @default.
• W4311873368 cites W2076495609 @default.
• W4311873368 cites W2078801689 @default.
• W4311873368 cites W2083210775 @default.
• W4311873368 cites W2092831145 @default.
• W4311873368 cites W2120688918 @default.
• W4311873368 cites W2120896997 @default.
• W4311873368 cites W2120962670 @default.
• W4311873368 cites W2125211371 @default.
• W4311873368 cites W2132946599 @default.
• W4311873368 cites W2136986799 @default.
• W4311873368 cites W2140289284 @default.
• W4311873368 cites W2147427406 @default.
• W4311873368 cites W2150652574 @default.
• W4311873368 cites W2154672407 @default.
• W4311873368 cites W2170000318 @default.
• W4311873368 cites W2419524464 @default.
• W4311873368 doi "https://doi.org/10.1101/2022.12.16.520769" @default.
• W4311873368 hasPublicationYear "2022" @default.
• W4311873368 type Work @default.
• W4311873368 citedByCount "0" @default.
• W4311873368 crossrefType "posted-content" @default.
• W4311873368 hasAuthorship W4311873368A5000551183 @default.
• W4311873368 hasAuthorship W4311873368A5008515166 @default.
• W4311873368 hasAuthorship W4311873368A5018736862 @default.
• W4311873368 hasAuthorship W4311873368A5025229383 @default.
• W4311873368 hasAuthorship W4311873368A5069150690 @default.
• W4311873368 hasAuthorship W4311873368A5075897372 @default.
• W4311873368 hasBestOaLocation W43118733681 @default.
• W4311873368 hasConcept C104317684 @default.
• W4311873368 hasConcept C11804247 @default.
• W4311873368 hasConcept C153911025 @default.
• W4311873368 hasConcept C174510640 @default.
• W4311873368 hasConcept C174851938 @default.
• W4311873368 hasConcept C179247698 @default.
• W4311873368 hasConcept C181199279 @default.
• W4311873368 hasConcept C187882448 @default.
• W4311873368 hasConcept C189482833 @default.
• W4311873368 hasConcept C2776634448 @default.
• W4311873368 hasConcept C2777576037 @default.
• W4311873368 hasConcept C2779222958 @default.
• W4311873368 hasConcept C54009773 @default.
• W4311873368 hasConcept C54355233 @default.
• W4311873368 hasConcept C55493867 @default.
• W4311873368 hasConcept C70721500 @default.
• W4311873368 hasConcept C71095615 @default.
• W4311873368 hasConcept C71829478 @default.
• W4311873368 hasConcept C86803240 @default.
• W4311873368 hasConcept C95444343 @default.
• W4311873368 hasConceptScore W4311873368C104317684 @default.
• W4311873368 hasConceptScore W4311873368C11804247 @default.
• W4311873368 hasConceptScore W4311873368C153911025 @default.
• W4311873368 hasConceptScore W4311873368C174510640 @default.
• W4311873368 hasConceptScore W4311873368C174851938 @default.
• W4311873368 hasConceptScore W4311873368C179247698 @default.
• W4311873368 hasConceptScore W4311873368C181199279 @default.
• W4311873368 hasConceptScore W4311873368C187882448 @default.
• W4311873368 hasConceptScore W4311873368C189482833 @default.
• W4311873368 hasConceptScore W4311873368C2776634448 @default.
• W4311873368 hasConceptScore W4311873368C2777576037 @default.
• W4311873368 hasConceptScore W4311873368C2779222958 @default.
• W4311873368 hasConceptScore W4311873368C54009773 @default.
• W4311873368 hasConceptScore W4311873368C54355233 @default.
• W4311873368 hasConceptScore W4311873368C55493867 @default.
• W4311873368 hasConceptScore W4311873368C70721500 @default.
• W4311873368 hasConceptScore W4311873368C71095615 @default.
• W4311873368 hasConceptScore W4311873368C71829478 @default.
• W4311873368 hasConceptScore W4311873368C86803240 @default.
• W4311873368 hasConceptScore W4311873368C95444343 @default.
• W4311873368 hasLocation W43118733681 @default.
• W4311873368 hasOpenAccess W4311873368 @default.
• W4311873368 hasPrimaryLocation W43118733681 @default.
• W4311873368 hasRelatedWork W1999116544 @default.
• W4311873368 hasRelatedWork W2354082017 @default.

• next